| Yazarlar |
Parham Taslimi
Türkiye |
Doç. Dr. Hatice Esra DURAN
Türkiye |
|
Yeliz Demir
Ardahan Üniversitesi, Türkiye |
|
Necla Öztaşkın
Türkiye |
|
Ahmet Maraş
Atatürk Üniversitesi, Türkiye |
|
İlhami Gülçin
Atatürk Üniversitesi, Türkiye |
|
Şükrü Beydemir
Anadolu Üniversitesi, Türkiye |
|
Süleyman Göksu
Atatürk Üniversitesi, Türkiye |
| Özet |
| Diabetes mellitus (DM) is a chronic metabolic disease in which there are high blood sugar levels over a prolonged period. Aldose reductase (AR) belongs to aldo-keto reductase superfamily and plays a key role in the polyol pathway. α-Glycosidase and α-amylase are important enzymes in glucose metabolism. In this study, AR was purified from purified from cow liver. The enzyme was obtained with 139.17 purification fold and with a specific activity of 1.67 EU/mg protein. Then, it is observed the inhibition effect of diarylmethanons (1a-d), bromophenols (2a-d and 4a-d) and diarylmethanes (3a-d) on aldose reductase, α-glycosidase and α-amylase enzymes. In these series, compound 2a showed lowest inhibitory activity against AR with a K value of 1.09 ± 0.29 μM while compound 2d showed highest inhibitory activity against AR with a K value of 0.092 ± 0.015 μM. Additionally, α-glycosidase and α-amylase enzymes were easily inhibited by these compounds. All compounds were tested for their inhibition effects against of α-glycosidase enzyme and demonstrated efficient inhibition profiles with K values in the range of 14.44 ± 0.88-43.53 ± 9.06 nM, and IC values in the range of 11.72-46.11 nM. Also, inhibition of the α-amylase enzyme, which determined an effective inhibition profile with IC values, is in the range of 3.84-29.61 nM. |
| Anahtar Kelimeler |
| alpha-Amylase | alpha-Glycosidase | Aldose reductase | Bromophenols | Diarylmethanons |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES |
| Dergi ISSN | 0141-8130 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 11-2018 |
| Cilt No | 119 |
| Sayfalar | 857 / 863 |
| Doi Numarası | 10.1016/j.ijbiomac.2018.08.004 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S0141813018328460 |
| Atıf Sayıları | |
| WoS | 171 |
| Google Scholar | 193 |
