Phenolic compounds: The inhibition effect on polyol pathway enzymes
Yazarlar (2)
Doç. Dr. Hatice Esra DURAN Türkiye
Prof. Dr. Şükrü Beydemir Anadolu Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | CHEMICO-BIOLOGICAL INTERACTIONS (Q1) | ||
| Dergi ISSN | 0009-2797 Dergi Bilgileri (2017) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2017 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-03-2017 |
| Cilt / Sayı / Sayfa | 266 / 0 / 47–55 | DOI | 10.1016/j.cbi.2017.01.021 |
| Makale Linki | http://dx.doi.org/10.1016/j.cbi.2017.01.021 | ||
| UAK Araştırma Alanları |
Tıbbi Biyokimya
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| Özet |
| The polyol pathway called as sorbitol way is a small part of glycose metabolism. The pathway is regarded as an important element in the pathogenesis of various diabetic complications in individuals with diabetes mellitus. The pathway plays a crucial role in hyperglycemia. The polyol pathway contains two enzymes as aldose reductase (AR) and sorbitol dehydrogenase (SDH). In the present study, AR and SDH were purified from sheep liver by using simple chromatographic methods. AR was purified with a yield of 2.11% and approximately 162 fold and SDH was purified with a yield of 0.53% and approximately 9.07 purification fold. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed to check the purity and determine the subunit molecular weights of the enzymes. Subunit molecular weights of AR and SDH were 38.82 kDa and 37.74 kDa, respectively. Optimal pH, optimal ionic … |
| Anahtar Kelimeler |
| Aldose reductase | Sorbitol dehydrogenase | Sheep liver | Phenolic acid | Inhibition |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 50 |
| Google Scholar | 70 |