Investigation of Campylobacteriosis in Abort Cases in Kars Province by Pathological, Immunohistochemical, PCR and Microbiological Methods   
Yazarlar (8)
Doç. Dr. Emin KARAKURT Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Hilmi NUHOĞLU Kafkas Üniversitesi, Türkiye
Prof. Dr. Serpil DAĞ Kafkas Üniversitesi, Türkiye
Prof. Dr. Aliye GÜLMEZ SAĞLAM Kafkas Üniversitesi, Türkiye
Prof. Dr. Enver BEYTUT Kafkas Üniversitesi, Türkiye
Prof. Dr. Mitat ŞAHİN Kafkas Üniversitesi, Türkiye
Prof. Dr. Salih OTLU Kafkas Üniversitesi, Türkiye
Prof. Dr. Özgür ÇELEBİ Kafkas Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale
Makale Alt Türü Diğer hakemli uluslarası dergilerde yayınlanan tam makale
Dergi Adı Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Dergisi
Dergi ISSN 2148-6239
Dergi Tarandığı Indeksler DOAJ, CAB Abstract, CiteFactor, Google Scholar, Science Library Index, International Institute of Organized Research, Researchbib, SciLit, SJIFactor, COSMOS IF, SOBIAD, Zoological Record
Makale Dili İngilizce
Basım Tarihi 01-2020
Cilt No 5
Sayı 2
Sayfalar 69 / 74
DOI Numarası 10.24880/maeuvfd.673903
Makale Linki https://dergipark.org.tr/tr/pub/maeuvfd/issue/55084/673903
Özet
Campylobacteriosis is an infectious, zoonotic infection characterized by offspring and infertility, leading to economic losses in cattle and sheep breeding. In this study, we aimed to investigate the incidence of cattle and sheep abortion in Kars region in terms of Campylobacteriosis and evaluate the results by PCR, immunohistochemical, histopathological and microbiological methods. In this context, liver and lung tissue examples and abomasum contents of 444 abort cases brought from Kars Center and districts to Kafkas University Faculty of Veterinary Medicine Pathology Department between 2013-2019 years were examined. Tissue examples from animals were fixed in % 10 buffered formaldehyde solutions. After routine procedures, paraffin blocks were prepared and sections with a thickness of 5 μm were taken for Hematoxylin & Eosin staining and 4 μm were taken for immunohistochemical staining . Sections were examined under light microscope to determine histopathologic changes. Organs belonging to aborted fetuses and abomasum contents were inoculated into the Preston Campylobacter Enrichment Broth containing microbial study selective supplement, and then enriched by pre-enrichment and then passed through Preston Campylobacter Selective Agar. Cultures in which the culture was incubated after incubation were examined for colony morphology and microscopic appearance and Campylobacter spp. suspicious colonies were evaluated by biochemical tests. As a result of histopathologic studies, characteristically, 7 of 17 abortion cases with multifocal necrotic hepatitis pattern and yellow abomasum contents were blurred and …
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