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Sığır melanomlarında S100, Vimentin, PCNA, p53 ve MMP-9’un immunohistokimyasal ekspresyonları    
Yazarlar (9)
Doç. Dr. Emin KARAKURT Doç. Dr. Emin KARAKURT
Türkiye
Doç. Dr. Uğur AYDIN Doç. Dr. Uğur AYDIN
Kafkas Üniversitesi, Türkiye
Prof. Dr. Engin KILIÇ Prof. Dr. Engin KILIÇ
Kafkas Üniversitesi, Türkiye
Prof. Dr. Enver BEYTUT Prof. Dr. Enver BEYTUT
Kafkas Üniversitesi, Türkiye
Prof. Dr. Serpil DAĞ Prof. Dr. Serpil DAĞ
Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Hilmi NUHOĞLU Dr. Öğr. Üyesi Hilmi NUHOĞLU
Türkiye
Uğur Yıldız
Türkiye
Arş. Gör. Ayfer YILDIZ UYSAL Arş. Gör. Ayfer YILDIZ UYSAL
Türkiye
Emre Kurtbaş
Türkiye
Devamını Göster
Özet
This study aimed to evaluate the expression of PCNA, p53, MMP-9, Vimentin and S100 immunohistochemically and determine the aggressiveness in diagnosis of bovine melanomas. The material of this study consisted of melanoma biopsy samples taken from 10 cattle brought to our department. Tissue samples from cattle were fixed in 10% buffered formalin solution. After routine procedures paraffin blocks were cut sections of 5 μm thickness. For bleaching, some heavily pigmented tumour sections were deparaffinized, hydrated, and incubated in 10% solution of hydrogen peroxide (H2O2) for 5 hours at 65oC until sections appeared clear and Hematoxylin & Eosin (H&E) staining was applied to the sections to detect histopathological changes. Sections were examined and photographed under a light microscope. Avidin-Biotin Peroxidase was used as the immunohistochemical method. We observed that the tumoral mass was solitary, firm, hairless, oval-round shaped and quite large. We detected spindle and epithelioid type tumoral cells containing a lot of large brownish-black granular melanin pigments in their cytoplasm. All melanoma cases were immune positive for S100, Vimentin, PCNA, p53 and MMP-9 expressions. In conclusion, we think that these immunohistochemical markers are quite convenient in evaluating the prognosis and diagnosis of bovine melanomas.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayınlanan tam makale
Dergi Adı Harran Üniversitesi Veteriner Fakültesi Dergisi
Dergi ISSN 2146-717X
Dergi Tarandığı Indeksler TR DİZİN
Makale Dili Türkçe
Basım Tarihi 03-2021
Cilt No 10
Sayı 1
Sayfalar 43 / 49
Doi Numarası 10.31196/huvfd.859433
Makale Linki http://dx.doi.org/10.31196/huvfd.859433