| Yazarlar |
Burcu Yücel
İstanbul Medeniyet Üniversitesi, Türkiye |
|
Ceren Sümer
Türkiye |
Prof. Dr. İlhami GÖK
Kafkas Üniversitesi, Türkiye |
|
Murat Karkucak
Karadeniz Teknik Üniversitesi, Türkiye |
|
Emel Alemdaroğlu
Türkiye |
|
Fahri Uçar
Akdeniz Üniversitesi, Türkiye |
| Özet |
| Various cytokine polymorphisms have been associated with genetic risk factors predisposing to Rheumatoid Arthritis (RA) in different populations. To predict the clinical outcome as well as response to therapy in RA, studies aimed to describe genetic markers. The present study aims to search for polymorphisms of 13 cytokine coding genes in the Eastern Black Sea Region of Turkey. DNAs of 49 patients and 96 healthy bone marrow and kidney donors were isolated from peripheral blood samples. Genotyping was performed using the Heidelberg Cytokine Typing Tray kit. PCR products were visualized on an agarose gel, and results were analyzed using the interpretation scheme provided with the kit. Arlequin 3.5 software was used for statistical analysis. No positive association was found between allele frequencies and the disease. However, a negative association was found for the IL-A -889 C allele (p=0.02, OR=0.533, Wald's 95% CI=0.318-0.893). IL-12 -1188 CC (p=0.01, OR=3.667, Wald's 95% CI=1.246-10.786), IL-4 -1098 GT (p=0.02, OR=2.405, Wald's 95% CI=1.129-5.125) genotypes were found positively associated with the RA, while IL-4 -590 CT (p=0.02, OR=0.422, Wald's 95% CI=0.201-0.886) was found negatively associated with the disease. In addition, IL-6 GG haplotype was found positively associated with the RA (p=0.02, OR=1.880, Wald's 95% CI=1.086-3.254). Our findings suggest that some polymorphisms of the IL-1A, IL-2, IL-4, IL-6 and IL-12 could be responsible for the susceptibility or protective to RA in our study population. Multi-centered and large numbers of subjects containing studies that search for cytokine polymorphisms will gather more information regarding the susceptibility to RA of Turkish patients. |
| Anahtar Kelimeler |
| Cytokine polymorphism | PCR-SSP | rheumatoid arthritis |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | NORTHERN CLINICS OF ISTANBUL |
| Dergi ISSN | 2148-4902 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2020 |
| Cilt No | 7 |
| Sayı | 6 |
| Sayfalar | 563 / 571 |
| Doi Numarası | 10.14744/nci.2020.70845 |
| Makale Linki | http://www.nclin.ist/jvi.aspx?un=NCI-70845volume= |
| Atıf Sayıları | |
| WoS | 8 |
