Pattern of RAD51-G135C Polymorphism among Eastern Mediterranean Turkish Population and Association with Breast Cancer
Yazarlar (2)
Prof. Dr. İlhami GÖK
Kafkas Üniversitesi, Türkiye
Süleyman Çetinkünar
Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | CYTOLOGY AND GENETICS | ||
| Dergi ISSN | 0095-4527 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2021 |
| Cilt / Sayı / Sayfa | 55 / 2 / 171–176 | DOI | 10.3103/S0095452721020043 |
| Özet |
| The RAD51 gene encodes proteins that are important for the repair of double-strand DNA breaks by recombination. Thus, genetic variability in the gene may contribute to the occurrence and progression of breast carcinoma. We investigated the association of polymorphisms in the DNA repair genes RAD51-site 135G/C with the breast cancer risk. Genotypes were determined by PCR-RFLP assays in 55 female patients with breast cancers and 80 age-matched healthy controls. Bloods samples were taken from all the patients and health controls and DNA was isolated. The region of interest was amplified based on PCR. After amplification, we used a restriction enzyme (RAD51; MvaI) and digested the PCR product. Then, these DNA fragments were size separated in gel electrophoresis. We identified changes in the nucleotides in these specific regions and confirmed the genotyping via sequencing a subset of PCR … |
| Anahtar Kelimeler |
| RAD51 gene | polymorphism | MvaI | breast cancer | eastern Mediterranean | Turkish population |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 1 |