The effect of polymorphism in DNA repair genes RAD51 and XRCC2 in colorectal cancer in Turkish population
Yazarlar (7)
Suleyman Cetinkunar
Prof. Dr. İlhami GÖK Kafkas Üniversitesi, Türkiye
Ruchan Bahadır Celep
Dogan İlhan
Hasan Erdem
Bulent Caglar Bilgin
Recep Aktimur
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | ||
| Dergi ISSN | 1940-5901 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2015 |
| Cilt / Sayı / Sayfa | 8 / 2 / 2649–2655 | DOI | – |
| Makale Linki | http://www.ijmeg.org | ||
| UAK Araştırma Alanları |
Tıbbi Genetik
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| Özet |
| The genes RAD51 and XRCC2 encode proteins that are important for the repair of double-strand DNA breaks by recombination. Therefore, genetic variability in these genes may contribute to the occurrence and progression of carcinoma. We investigated the association of polymorphisms in the DNA repair genes XRCC2-A/G and RAD51-135G/C with the colorectal cancer risk. Genotypes were determined by PCR-RFLP assays in 71 patients with colorectal cancer and 86 age-matched healthy controls. After amplification, we used a restriction enzyme (RAD51; MvaI and XRCC2; HphI) and digested the PCR product. Then, this DNA fragments were passed through gel electrophoresis. By examining these images, we identified changes in the nucleotides in these specific regions. To clarify fragments polymorphisms, the PCR products were sequenced with an Applied Biosystems Automated Sequencer. We observed … |
| Anahtar Kelimeler |
| Colon cancer | RAD51 | XRCC2 genes | polymorphism | Turkey |
