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Pathological and Biochemical Investigation of the Effects of L-Carnitine and Gemfibrozilin, Peroxisome Proliferator Activated Receptors (PPARS), Oxidative Stress and Lipidosis in High Fat Diet Rabbits    
Yazarlar (10)
Mehmet Çitil
Türkiye
Recai Tunca
Türkiye
Erdoğan Uzlu
Türkiye
Prof. Dr. Mahmut KARAPEHLİVAN Prof. Dr. Mahmut KARAPEHLİVAN
Kafkas Üniversitesi, Türkiye
Kürşad Yapar
Türkiye
Yasemen Adalı
Türkiye
Hüseyin Avni Eroğlu
Türkiye
Doç. Dr. Ekin Emre ERKILIÇ Doç. Dr. Ekin Emre ERKILIÇ
Türkiye
Doç. Dr. Mustafa MAKAV Doç. Dr. Mustafa MAKAV
Türkiye
Hidayet Metin Erdoğan
Türkiye
Devamını Göster
Özet
Obesity and fatty liver is a worldwide health problem in human with detrimental consequences where many investigations are undertaken to overcome this problem. In this study, gemfibrozil and L-carnitine were evaluated in prevention of obesity and lipidosis. The study involved 56 New-Zealand Albino rabbits, divided into 8 equal groups (n=7). The groups were as follow; group I (normal diet), II (normal diet +gemfibrozil), III (normal diet+L-carnitine) and IV (normal diet+gemfibrozil+L- carnitine), V (high fat diet), VI (high fat diet+gemfibrozil), VII (high fat diet+L- carnitine) and VIII (high fat diet+gemfibrozil+L-carnitine). Animals were blood sampled and wieght weekly during the experiment and at the end of the experiment for determination of biochemical parameters (glucose, total lipid). All rabbits were euthanised for histopathological examination and for distrubition of peroxisome proliferator activated receptors (PPARs) in tissues by immunohystochemistry. Gemfibrozil and L-carnitin treatment in rabbits given high fat diet resulted in statistically significant decrease in total lipid when compared to those only received high fat diet. Beta oxidation of high fat diet group was significantly higher than that of groups additionally received gemfibrozil and L-carnitine. Immunohistochemistry revealed an increase in PPAR, PPAR-α and β but not PPAR-γ expression in high fat diet group. On the contrary, L-carnitin administration had no effect on tissue PPAR expression. PPAR-α expression differed between groups received gemfibrozil and high fat diet and those did not. The most marked macroscopy finding was abdominal fat increase in high fat diet group (group V). On the other hand gemfibrozil administration resulted in significant abdominal fat decrease. Furthermore decreased abdominal fat was marked in gemfibrozil and L-carnitine given animals (group VIII) when compared to other groups. In conclusion, gemfibrozil and L-carnitine administration alleviated abdominal and hepatic fattening. Gemfibrozil also caused a significant increase in PPAR-α expression in the liver. It may be of use in avoiding abdominal fat (obesity) due to high fat diet by use of gemfibrozil, a synthetic PPAR-a ligand, and L-carnitine.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayımlanan tam makale
Dergi Adı Journal of Advances in VetBio Science and Techniques
Dergi ISSN 2548-1150
Dergi Tarandığı Indeksler TR DİZİN (ULAKBİM)
Makale Dili Türkçe
Basım Tarihi 12-2022
Cilt No 7
Sayı 3
Sayfalar 346 / 360
Doi Numarası 10.31797/vetbio.1136444
Makale Linki http://dx.doi.org/10.31797/vetbio.1136444