img
Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts   
Yazarlar
Doç. Dr. Doğan AKÇA
Türkiye
Prof. Dr. Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye
Emre Karakaya
Türkiye
Dr. Öğr. Üyesi Mustafa Reha COŞKUN
Türkiye
Dr. Öğr. Üyesi Elif ÇELİK
Kafkas Üniversitesi, Türkiye
Prof. Dr. Mitat ŞAHİN
Kafkas Üniversitesi, Türkiye
Prof. Dr. Özgür ÇELEBİ
Türkiye
Prof. Dr. Salih OTLU
Türkiye
Doç. Dr. Aliye GÜLMEZ SAĞLAM
Türkiye
Arş. Gör. Eray BÜYÜK
Türkiye
Arş. Gör. Seda GÖKDEMİR
Türkiye
İzzet Burçin Satıcıoğlu
Türkiye
Seçil Abay
Türkiye
Tuba Kayman
Türkiye
Fuat Aydın
Türkiye
Özet
Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to characterize Bacillus anthracis isolates recovered from a variety of hosts and environments during anthrax sporadic in the Kars and Kayseri regions of Turkey. For this purpose, a total of 23 B. anthracis field isolates obtained from human, cattle, sheep, dog, horse, puma, soil, and fodder were used. In addition, the Sterne vaccine strain was included. All B. anthracis isolates were confirmed via molecular tests using plasmid-based PCR and 16S rRNA gene sequencing. All were found to be positive for protective antigen (PA) and capsule (Cap) genes. The presence of two specific 16S rRNA positions (1139 and 1148) with nucleotide mismatches was used to differentiate between B. anthracis species in the Bacillus cereus group, which has a relatively high (>99%) 16S rRNA sequence similarity. All of our isolates, including the Sterne vaccine strain, were classified in the same 16S rRNA type (Type 6) that has been reported previously as being the predominant type for B. anthracis isolates.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Turkish Journal of Veterinary and Animal Sciences
Dergi ISSN 1728-1997
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2022
Cilt No 46
Sayı 1
Sayfalar 44 / 51
Doi Numarası 10.3906/vet-2106-111
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
TRDizin 1
Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts

Paylaş