| Yazarlar |
Doç. Dr. Murat KARAMEŞE
Kafkas Üniversitesi, Türkiye |
Hüseyin Serkan Erol
Kastamonu Üniversitesi, Türkiye |
|
Mevlüt Albayrak
Atatürk Üniversitesi, Türkiye |
|
Gülname Fındık Güvendi
Recep Tayyip Erdoğan Üniversitesi, Türkiye |
|
Emsal Aydın
Giresun Üniversitesi, Türkiye |
Prof. Dr. Selina AKSAK KARAMEŞE
Kafkas Üniversitesi, Türkiye |
| Özet |
| We hypothesize that apigenin may inhibit some cellular process of sepsis-induced spleen injury and simultaneously improve inflammation and oxidative stress. Therefore, the aim of this study was to investigate the potential protective effects of apigenin in a polymicrobial sepsis rat model of by cecal ligation and puncture. 64 female Wistar albino rats were divided into 8 groups. The pro-inflammatory (tumor necrosis factor-alpha, interleukin-6, and interleukin-1-beta) and anti-inflammatory (tumor growth factor-beta and interleukin-10) cytokine levels were measured by enzyme-linked immunosorbent assay. CD3, CD68, and nuclear factor kappa B (NF-κB) positivity rates were detected by immunohistochemical methods. Oxidative stress parameters were measured by tissue biochemistry. Sepsis caused a significant increase in TNF-alpha, IL-1-beta, IL-6, and TGF-beta levels whereas it reduced IL-10 level. Additionally, it led to an increase in CD3, CD68, and NF-κB positivity rates as well as oxidative stress parameters levels. However, apigenin inhibited the inflammation process, increased the IL-10 level and normalized the oxidative stress parameters. Pretreatment with apigenin results in a significant reduction in the amount of inflammatory cells. The beneficial effect of apigenin on spleen injury also involved inhibition of NF-κB pathway, suppression of proinflammatory cytokines, and induction of anti-inflammatory cytokine production. Additionally, it led to a decrease in oxidative stress in spleen tissue. Taking everything into account, apigenin may be an alternative therapeutic option for prevention of sepsis-induced organ. |
| Anahtar Kelimeler |
| Apigenin | CD3 and CD68 | CLP sepsis | cytokines | NF-kappa B | oxidative stress |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY |
| Dergi ISSN | 0892-3973 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 06-2016 |
| Cilt No | 38 |
| Sayı | 3 |
| Sayfalar | 228 / 237 |
| Doi Numarası | 10.3109/08923973.2016.1173058 |
| Makale Linki | http://www.tandfonline.com/doi/full/10.3109/08923973.2016.1173058 |
| Atıf Sayıları | |
| WoS | 52 |
