Anti-oxidant and anti-inflammatory effects of apigenin in a rat model of sepsis: an immunological, biochemical, and histopathological study
Yazarlar (6)
Doç. Dr. Murat KARAMEŞE
Kafkas Üniversitesi, Türkiye
Hüseyin Serkan Erol
Kastamonu Üniversitesi, Türkiye
Mevlüt Albayrak
Atatürk Üniversitesi, Türkiye
Gülname Fındık Güvendi
Recep Tayyip Erdoğan Üniversitesi, Türkiye
Emsal Aydın
Giresun Üniversitesi, Türkiye
Prof. Dr. Selina AKSAK KARAMEŞE
Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (Q2) | ||
| Dergi ISSN | 0892-3973 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 06-2016 |
| Cilt / Sayı / Sayfa | 38 / 3 / 228–237 | DOI | 10.3109/08923973.2016.1173058 |
| Makale Linki | http://www.tandfonline.com/doi/full/10.3109/08923973.2016.1173058 | ||
| Özet |
| We hypothesize that apigenin may inhibit some cellular process of sepsis-induced spleen injury and simultaneously improve inflammation and oxidative stress. Therefore, the aim of this study was to investigate the potential protective effects of apigenin in a polymicrobial sepsis rat model of by cecal ligation and puncture. 64 female Wistar albino rats were divided into 8 groups. The pro-inflammatory (tumor necrosis factor-alpha, interleukin-6, and interleukin-1-beta) and anti-inflammatory (tumor growth factor-beta and interleukin-10) cytokine levels were measured by enzyme-linked immunosorbent assay. CD3, CD68, and nuclear factor kappa B (NF-κB) positivity rates were detected by immunohistochemical methods. Oxidative stress parameters were measured by tissue biochemistry. Sepsis caused a significant increase in TNF-alpha, IL-1-beta, IL-6, and TGF-beta levels whereas it reduced IL-10 level. Additionally, it led to an increase in CD3, CD68, and NF-κB positivity rates as well as oxidative stress parameters levels. However, apigenin inhibited the inflammation process, increased the IL-10 level and normalized the oxidative stress parameters. Pretreatment with apigenin results in a significant reduction in the amount of inflammatory cells. The beneficial effect of apigenin on spleen injury also involved inhibition of NF-κB pathway, suppression of proinflammatory cytokines, and induction of anti-inflammatory cytokine production. Additionally, it led to a decrease in oxidative stress in spleen tissue. Taking everything into account, apigenin may be an alternative therapeutic option for prevention of sepsis-induced organ. |
| Anahtar Kelimeler |
| Apigenin | CD3 and CD68 | CLP sepsis | cytokines | NF-kappa B | oxidative stress |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 62 |