| Yazarlar |
Murat KARAMEŞE
Kafkas Üniversitesi, Türkiye |
Bülent Güvendi
Türkiye |
Selina AKSAK KARAMEŞE
Kafkas Üniversitesi, Türkiye |
|
İrfan Çınar
Türkiye |
Serpil CAN
Kafkas Üniversitesi, Türkiye |
|
Hüseyin Serkan Erol
Kastamonu Üniversitesi, Türkiye |
|
Hakan Aydın
Atatürk Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Volkan GELEN
Kafkas Üniversitesi, Türkiye |
|
Emre Karakuş
Türkiye |
| Özet |
| In the present study, our aim was to investigate the possible protective effects of epigallocatechin gallate (EGCG) on lipopolysaccharide (LPS)-induced hepatotoxicity by using Hep3B human hepatoma cells. Specifically, the study examines the role of some proinflammatory markers and oxidative damage as possible mechanisms of LPS-associated cytotoxicity. Consequently, the hepatocellular carcinoma cell line Hep3B was chosen as a model for investigation of LPS toxicity and the effect of EGCG on LPS-exposed cells. The Hep3B human hepatoma cells were used for this study. The cytotoxic effects of chemicals (EGCG and LPS), AST and ALT levels, SOD and CAT activities, GSH-Px level and TNF-alpha and IL-6 levels were detected by using different biochemical and molecular methods. LPS and EGCG were applied to cells at various times and doses. The highest treatment dose of EGCG (400 µM) led to a dramatic decrease in SOD level and increase in CAT and GSH levels. Additionally, the highest dose of EGCG also led to a dramatic increase in TNF-alpha and IL-6 levels. On the other hand, effective doses of EGCG (200 and 100 µM) normalized all related parameters levels. LPS caused hepatotoxicity, but interestingly, a high dose of EGCG was found to be a cytotoxic agent in this study. However, other two doses of EGCG led to a decrease in both inflammatory cytokine levels and antioxidant enzyme levels. Further studies should examine the effect of EGCG on secondary cellular signaling pathways. |
| Anahtar Kelimeler |
| Cytokines | Epigallocatechin gallate | Hep3B | Lipopolysaccharide | Oxidative stress |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES |
| Dergi ISSN | 2008-3866 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 05-2016 |
| Cilt No | 19 |
| Sayı | 5 |
| Sayfalar | 483 / 489 |
