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Molecular detection of Papillomavirus and immunohistochemical investigation of p53 gene expressions in bovine papillomas and fibropapillomas        
Yazarlar
Doç. Dr. Emin KARAKURT Doç. Dr. Emin KARAKURT
Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Nüvit COŞKUN Dr. Öğr. Üyesi Nüvit COŞKUN
Türkiye
Prof. Dr. Serpil DAĞ Prof. Dr. Serpil DAĞ
Kafkas Üniversitesi, Türkiye
Prof. Dr. Enver BEYTUT Prof. Dr. Enver BEYTUT
Kafkas Üniversitesi, Türkiye
Veysel Soydal Ataseven
Türkiye
Prof. Dr. Volkan YILMAZ Prof. Dr. Volkan YILMAZ
Türkiye
Fırat Doğan
Türkiye
Dr. Öğr. Üyesi Hilmi NUHOĞLU Dr. Öğr. Üyesi Hilmi NUHOĞLU
Kafkas Üniversitesi, Türkiye
Prof. Dr. Celal Şahin ERMUTLU Prof. Dr. Celal Şahin ERMUTLU
Türkiye
Doç. Dr. Uğur AYDIN Doç. Dr. Uğur AYDIN
Kafkas Üniversitesi, Türkiye
Doç. Dr. Mushap KURU Doç. Dr. Mushap KURU
Kafkas Üniversitesi, Türkiye
Arş. Gör. Ayfer YILDIZ UYSAL Arş. Gör. Ayfer YILDIZ UYSAL
Kafkas Üniversitesi, Türkiye
Özet
Papilloma and fibropapilloma cases are quite common in cattle breeding, which cause economic losses due to decrease in the production of milk, meat, and also impair the quality of hide. In this study, we aimed to determine viral etiology and investigate p53 expression levels with immunohistochemical methods from a total of 30 cases. The study material was collected between 2013 and 2021 in Kars, Turkey. Paraffin embedded tissues were used for earlier cases in which the freshly specimens could not be provided. Cases were investigated for papillomavirus etiology with polymerase chain reaction (PCR) using FAP59/FAP64 and MY09/MY11 primer pairs. In 20 of the 30 cases papillomaviruses were identified, and 10 cases were identified as Bovine papillomavirus-1 (BPV-1), 1 case as BPV-2, 1 case as BPV-12, and 1 case as equus caballus papillomavirus 2 (EcPV-2) after sequence analysis. p53 immunostaining was also performed, and the cases were graded according to the positively stained cells. In conclusion BPV-12 was detected for the first time in our country, EcPV-2 was detected first time in cattle indicating cross species infection and p53 was staining most evident in BPV-1 and BPV-2 cases and BPV-12 and EcPV-2 was not stained.
Anahtar Kelimeler
Cattle | Immunohistochemistry | Papilloma | Polymerase chain reaction | p53
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı ARCHIVES OF MICROBIOLOGY
Dergi ISSN 0302-8933
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q3
Makale Dili İngilizce
Basım Tarihi 05-2022
Cilt No 204
Sayı 5
Sayfalar 1 / 12
Doi Numarası 10.1007/s00203-022-02902-0
Makale Linki https://link.springer.com/article/10.1007/s00203-022-02902-0