| Yazarlar |
Prof. Dr. Duygu KAYA
Kafkas Üniversitesi, Türkiye |
Aykut Gram
Türkiye |
|
Marıusz Pawel Kowalewskı
Türkiye |
|
Sabine Schaefer Somı
Türkiye |
Doç. Dr. Mushap KURU
Kafkas Üniversitesi, Türkiye |
|
Aloıs Boos
Türkiye |
|
Selim Aslan
Yakın Doğu Üniversitesi, Türkiye |
| Özet |
| The goals of this study were as follows: (Experiment 1) to examine the basic capability of canine corpora lutea (CL) to respond to GnRH by assessing expression of gonadotropin-releasing hormone receptor (GnRH-R) in luteal samples collected throughout the luteal lifespan from non-pregnant dogs, and (Experiment 2) to investigate the effects of pre-pubertal application of the GnRH agonist deslorelin acetate on luteal function following the first oestrus. Mature CL were collected during the mid-luteal phase (days 30–45) from treated and control bitches. Transcript levels of several factors were determined: estrogen receptors (ESR1/ERα, ESR2/ERβ), progesterone (P4)-receptor (PGR), prolactin receptor (PRLR), PGE2-synthase (PTGES) and PGE2 receptors (PTGER2/EP2, PTGER4/EP4), vascular endothelial growth factor (VEGFA) and VEGF receptors (VEGFR1 and VEGFR2), cyclooxygenase 2 (COX2/PTGS2), steroidogenic acute regulatory protein (STAR) and 3β-hydroxysteroid dehydrogenase (3βHSD). Additionally, levels of Kisspeptin 1 (Kiss1) and its receptor (KISS1-R) were evaluated. Although generally low, GnRH-R expression was time dependent and was elevated during early dioestrus, with a significant decrease towards luteal regression. In deslorelin-treated and control dogs, its expression was either low or frequently below the detection limit. EP2 and VEGFR1 were higher in the treated group, which could be caused by a feedback mechanism after long-term suppression of reproductive activity. Despite large individual variations, 3βHSD was higher in the deslorelin-treated group. This, along with unchanged STAR expression, was apparently not mirrored in increased luteal functionality, because similar P4 levels were detected in both groups. Finally, the deslorelin-mediated long-term delay of puberty does not have negative carry-over effects on subsequent ovarian functionality in bitches. |
| Anahtar Kelimeler |
| deslorelin,domestic dog (Canis familiaris),GnRH receptor,luteal function,puberty delay |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Reproduction in Domestic Animals |
| Dergi ISSN | 0936-6768 |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2017 |
| Cilt No | 52 |
| Sayı | 6 |
| Sayfalar | 1104 / 1112 |
| Doi Numarası | 10.1111/rda.13038 |
| Makale Linki | http://doi.wiley.com/10.1111/rda.13038 |
| Atıf Sayıları | |
| WoS | 8 |
| SCOPUS | 8 |
| Google Scholar | 11 |
