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Molecular characterization of Bacillus anthracis isolates recovered from nomic and non-nomic hosts    
Yazarlar
 Doğan AKÇA Doğan AKÇA
Kafkas Üniversitesi, Türkiye
 Fatih BÜYÜK Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye
Emre Karakaya
Türkiye
Dr. Öğr. Üyesi Mustafa Reha COŞKUN Dr. Öğr. Üyesi Mustafa Reha COŞKUN
Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Elif ÇELİK Dr. Öğr. Üyesi Elif ÇELİK
Kafkas Üniversitesi, Türkiye
 Mitat ŞAHİN Mitat ŞAHİN
Kafkas Üniversitesi, Türkiye
 Özgür ÇELEBİ Özgür ÇELEBİ
Kafkas Üniversitesi, Türkiye
 Salih OTLU Salih OTLU
Kafkas Üniversitesi, Türkiye
 Aliye GÜLMEZ SAĞLAM Aliye GÜLMEZ SAĞLAM
Kafkas Üniversitesi, Türkiye
Arş. Gör. Eray BÜYÜK Arş. Gör. Eray BÜYÜK
Türkiye
Arş. Gör. Seda GÖKDEMİR Arş. Gör. Seda GÖKDEMİR
Türkiye
İzzet Burçin Satıcıoğlu
Türkiye
Seçil Abay
Türkiye
Tuba Kayman
Türkiye
Fuat Aydın
Türkiye
Özet
Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle
of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to
characterize Bacillus anthracis isolates recovered from a variety of hosts and environments during anthrax sporadic in the Kars and
Kayseri regions of Turkey. For this purpose, a total of 23 B. anthracis field isolates obtained from human, cattle, sheep, dog, horse, puma,
soil, and fodder were used. In addition, the Sterne vaccine strain was included. All B. anthracis isolates were confirmed via molecular
tests using plasmid-based PCR and 16S rRNA gene sequencing. All were found to be positive for protective antigen (PA) and capsule
(Cap) genes. The presence of two specific 16S rRNA positions (1139 and 1148) with nucleotide mismatches was used to differentiate
between B. anthracis species in the Bacillus cereus group, which has a relatively high (>99%) 16S rRNA sequence similarity. All of our
isolates, including the Sterne vaccine strain, were classified in the same 16S rRNA type (Type 6) that has been reported previously as
being the predominant type for B. anthracis isolates.
Anahtar Kelimeler
Bacillus anthracis | plasmid-based PCR | 16S rRNA sequencing | phylogeny
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
Dergi ISSN 1300-0128
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q4
Makale Dili Türkçe
Basım Tarihi 01-2022
Cilt No 46
Sayı 1
Sayfalar 44 / 51
Doi Numarası 10.3906/vet-2106-111
Makale Linki http://dx.doi.org/10.3906/vet-2106-111