Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts
   
Yazarlar (15)
Doç. Dr. Doğan AKÇA Kafkas Üniversitesi, Türkiye
Prof. Dr. Fatih BÜYÜK Kafkas Üniversitesi, Türkiye
Emre Karakaya Erciyes Üniversitesi, Türkiye
Dr. Öğr. Üyesi Mustafa Reha COŞKUN Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Elif ÇELİK Kafkas Üniversitesi, Türkiye
Prof. Dr. Mitat ŞAHİN Kafkas Üniversitesi, Türkiye
Prof. Dr. Özgür ÇELEBİ Kafkas Üniversitesi, Türkiye
Prof. Dr. Salih OTLU Kafkas Üniversitesi, Türkiye
Prof. Dr. Aliye GÜLMEZ SAĞLAM Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Eray BÜYÜK Kafkas Üniversitesi, Türkiye
İzzet Burçin Satıcıoğlu Erciyes Üniversitesi, Türkiye
Seçil Abay Erciyes Üniversitesi, Türkiye
Tuba Kayman Sağlık Bilimleri Üniversitesi, Türkiye
Fuat Aydın Erciyes Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES (Q4)
Dergi ISSN 1300-0128 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI
Makale Dili İngilizce Basım Tarihi 01-2022
Cilt / Sayı / Sayfa 46 / 1 / 44–51 DOI 10.3906/vet-2106-111
Makale Linki http://dx.doi.org/10.3906/vet-2106-111
Özet
Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle
of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to
characterize Bacillus anthracis isolates recovered from a variety of hosts and environments during anthrax sporadic in the Kars and
Kayseri regions of Turkey. For this purpose, a total of 23 B. anthracis field isolates obtained from human, cattle, sheep, dog, horse, puma,
soil, and fodder were used. In addition, the Sterne vaccine strain was included. All B. anthracis isolates were confirmed via molecular
tests using plasmid-based PCR and 16S rRNA gene sequencing. All were found to be positive for protective antigen (PA) and capsule
(Cap) genes. The presence of two specific 16S rRNA positions (1139 and 1148) with nucleotide mismatches was used to differentiate
between B. anthracis species in the Bacillus cereus group, which has a relatively high (>99%) 16S rRNA sequence similarity. All of our
isolates, including the Sterne vaccine strain, were classified in the same 16S rRNA type (Type 6) that has been reported previously as
being the predominant type for B. anthracis isolates.
Anahtar Kelimeler
Bacillus anthracis | plasmid-based PCR | 16S rRNA sequencing | phylogeny
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
WoS 3
TRDizin 1
Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts

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