| Yazarlar |
Prof. Dr. Mitat ŞAHİN
Kafkas Üniversitesi, Türkiye |
Prof. Dr. Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye |
Prof. Dr. Özgür ÇELEBİ
Kafkas Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Mustafa Reha COŞKUN
Kafkas Üniversitesi, Türkiye |
| Özet |
| A 6-year-old Simental-Swiss Brown cross-bred cow, which aborted in the first trimester of the gestation and involuntarily presented anthrax bacilli around the udder, was subjected to this study. The cow was vaccinated with an attenuated anthrax vaccine (Bacillus anthracis 34 F2) 6 months ago and had normal physical examination findings. Samples taken 10 days after the abortion were representative of all possible spaces that could carry the bacteria such as milk, blood, environment, and swabs from rectum, udder and tits of an infected cow. Microbiological examination was carried out by staining the smears with Azure and in vitro culture of the samples on some enriched and selective media such as 7% sheep blood agar (SBA), Brain Heart Infusion (BHI) Broth media with 7% sheep blood, and polymyxin-lysozyme-ethylenediaminetetraacetic acid-thallous acetate (PLET) agar. On a molecular basis, the virulence plasmid-targeted PCR analysis of the isolates was performed. In addition, MLVA-8 and MLVA-25 typing of B. anthracis isolates were conducted. Upon the isolation of B. anthracis with a concentration of 6 × 102 cfu/mL in milk sample and different subtypes obtained from the isolates by MLVA-8 and MLVA-25 typing, the present study enables us to instantiate the presence and unusual spread of bacteria around the udder of cows. |
| Anahtar Kelimeler |
| Bacillus anthracis,milk,udder,culture,PCR,MLVA |
| Makale Türü | Diğer (Teknik, not, yorum, vaka takdimi, editöre mektup, özet, kitap krıtiği, araştırma notu, bilirkişi raporu ve benzeri) |
| Makale Alt Türü | SCI, SSCI, AHCI, SCI-Exp dergilerinde yayımlanan teknik not, editöre mektup, tartışma, vaka takdimi ve özet türünden makale |
| Dergi Adı | TURKISH JOURNAL OF VETERINARY ANIMAL SCIENCES |
| Dergi ISSN | 1300-0128 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2020 |
| Cilt No | 44 |
| Sayfalar | 955 / 960 |
| Doi Numarası | 10.3906/vet-2002-48 |
| Atıf Sayıları | |
| WoS | 1 |
