Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)

Ufuk Kamber; Mükremin Özkan Arslan; Güven Gülbaz; Gencay Taşkın Taşçı; Atila Akça

doi:10.3906/vet-1705-84

Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)

Yazarlar
Prof. Dr. Ufuk KAMBER Kurum Bilgileri Veteriner Fakültesi Gıda Güvenliği ve Halk Sağlığı Veterinerlik Gıda Hijyeni ve Üretimi Özgeçmiş Sayfası İletişim Bilgileri: Kafkas Üniversitesi, Türkiye
Prof. Dr. Mükremin Özkan ARSLAN Kurum Bilgileri Tıp Fakültesi Temel Tıp Bilimleri Tıbbi Parazitoloji Özgeçmiş Sayfası İletişim Bilgileri: Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Güven GÜLBAZ Kurum Bilgileri Mühendislik-Mimarlık Fakültesi Gıda Mühendisliği Gıda Bilimleri Özgeçmiş Sayfası İletişim Bilgileri: Kafkas Üniversitesi, Türkiye
Prof. Dr. Gencay Taşkın TAŞÇI Kurum Bilgileri Veteriner Fakültesi Klinik Öncesi Bilimler Veterinerlik Parazitolojisi Özgeçmiş Sayfası İletişim Bilgileri: Kafkas Üniversitesi, Türkiye
Atila Akça Kafkas Üniversitesi, Türkiye

Özet

Sarcocystisspecies are the most commonly encountered parasitic organisms in animals.Sarcocystis hominis( Sarcocystisbovihominis ), the species found in beef cattle, is not visible macroscopically in muscle tissue. In recent years it has been establishedthat, in addition to being a zoonotic parasite transmitted via the consumption of beef, the parasite produces toxins and causes foodpoisoning. Therefore, this study was undertaken in order to determine the extent to whichSarcocystisspecies are present in mincedmeat, meatballs, and fermented sausages produced from beef and marketed in Kars. A total of 150 samples, 50 of each product, werecollected in the city center from butcher s shops, from businesses that produce such items, and from general stores. In order to detectsarcocysts in the samples, the cysts revealed by an enzymatic digestion test were examined microscopically. Genomic DNA extractedfrom the samples was stored at20 °C for analysis by polymerase chain reaction (PCR). The microscopic analysis revealed that theparasite was present in 2 samples of minced meat (4%) and 1 sample of meatballs (2%). Analysis by PCR revealed that the parasite waspresent in 14 samples of minced meat (28%), 34 samples of meatballs (68%), and 1 sample of sausage (2%). These results indicate that theuse of PCR in the routine analysis of meat preparations for the presence ofSarcocystisspecies should be increased, since the applicationof this technique would provide analysts with more conclusive results than examination by microscope.

Anahtar Kelimeler

Makale Türü	Özgün Makale
Makale Alt Türü	SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı	TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES
Dergi ISSN	1300-0128
Dergi Tarandığı Indeksler	SCI-Expanded
Dergi Grubu	Q4
Makale Dili	İngilizce
Basım Tarihi	01-2018
Cilt No	42
Sayfalar	1 / 6
Doi Numarası	10.3906/vet-1705-84
Makale Linki	http://online.journals.tubitak.gov.tr/openDoiPdf.htm?mKodu=vet-1705-84

Atıf Sayıları
TRDizin	2

Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)

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Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)

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