| Yazarlar |
Burcu Üstüner
Türkiye |
|
Hakan Üstüner
Türkiye |
|
Elif Gökçe
Tekirdağ Namık Kemal Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Nail Tekin ÖNDER
Kafkas Üniversitesi, Türkiye |
|
M Melih Yılmaz
Türkiye |
|
Oguzhan Huraydin
Türkiye |
|
Mehmed Berk Toker
Türkiye |
| Özet |
| This study aimed to determine how melatonin (MT) and seminal plasma affected the freezability of buck sperm during the nonbreeding season. Semen was collected from eight bucks before (pre-MT) and after (post-MT) MT application in the nonbreeding season. Individual ejaculates were collected from the bucks, split into two equal groups according to the removal of seminal plasma (SP) (-) or nonremoval of SP (+). For washing, the groups of ejaculates were centrifuged, and the supernatant was separated, SP (-) and SP (+) ejaculates were diluted, then frozen. Semen samples were examined for sperm motility, plasma membrane integrity, defective acrosomes, DNA fragmentation, and mitochondrial membrane function at the native and post-thaw stages. When the general average post-thaw motility ( < 0.01), plasma membrane ( < 0.05), acrosome ( < 0.05), and DNA integrity rates ( < 0.05) and mitochondrial membrane potential (MMP) ( < 0.01) were evaluated, it was seen that MT administration caused a statistically significant improvement. The dramatic effect of nonremoval of seminal plasma on motility and plasma membrane integrity is more clearly observed in individual semen samples frozen in the pre-MT group ( < 0.05). Also, it was observed that removing seminal plasma in the post-MT group caused even milder post-thaw acrosome damage compared with the SP (+) group ( < 0.05). The effect of removing seminal plasma was not observed in terms of DNA integrity and MMP rates in pre- and post-MT groups. As a result, it was concluded that MT application and removal of seminal plasma in the nonbreeding season result in improvement in the freezability of buck semen. |
| Anahtar Kelimeler |
| buck seminal plasma,melatonin implant,cryopreservation,mitochondrial membrane potential,DNA integrity |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Biopreservation and Biobanking |
| Dergi ISSN | 1947-5535 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | Türkçe |
| Basım Tarihi | 08-2023 |
| Cilt No | 21 |
| Doi Numarası | 10.1089/bio.2022.0074 |
| Makale Linki | http://dx.doi.org/10.1089/bio.2022.0074 |
| Atıf Sayıları | |
| WoS | 1 |
