Effects of alpha‐lipoic acid on ram semen cryopreservation and post‐thaw life span
Yazarlar (3)
Doç. Dr. Nail Tekin ÖNDER Kafkas Üniversitesi, Türkiye
Prof. Dr. Zekariya Nur Bursa Uludağ Üniversitesi, Türkiye
Prof. Dr. Selim Alçay Bursa Uludağ Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | ANDROLOGIA (Q2) | ||
| Dergi ISSN | 0303-4569 Dergi Bilgileri (2022) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 02-2022 |
| Kabul Tarihi | 10-09-2021 | Yayınlanma Tarihi | 22-09-2021 |
| Cilt / Sayı / Sayfa | 54 / 1 / 1–5 | DOI | 10.1111/and.14249 |
| Makale Linki | http://dx.doi.org/10.1111/and.14249 | ||
| UAK Araştırma Alanları |
Dölerme ve Suni Tohumlama
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| Özet |
| The aim of the present study was to evaluate the effects of an alpha-lipoic acid-supplemented extender on ram semen at a post-thaw stage and after incubation (6 hr). Semen samples were collected from five Kivircik Rams. Pooled semen was diluted with an egg yolk-based extender with different concentrations of alpha-lipoic acid (0.25 mmol L , 0.5 mmol L and 1 mmol L ) and without alpha-lipoic acid. Motility, plasma membrane functional integrity (HOST), acrosome integrity (FITC-Pisum sativum agglutinin) and DNA integrity (TUNEL) were assessed at post-thaw and 6 hr after incubation of the frozen-thawed semen. At the post-thaw stage, 0.25 mmol L alpha-lipoic acid had a positive effect on sperm motility and plasma membrane functional integrity compared to the control group (p < .05). At the post-incubation stage (6 hr), it was determined that the motility and plasma membrane functional integrity of the antioxidant groups were adversely affected compared to the control group (p < .05) and 1 mmol L dose of alpha-lipoic acid had a harmful effect on DNA integrity compared to the control group (p < .05). The results of the study demonstrated that alpha-lipoic acid has positive effects at post-thaw but have harmful effects on long term to ram semen. |
| Anahtar Kelimeler |
| alpha-lipoic acid | cryopreservation | incubation | ram semen |
| Atıf Sayıları | |
| Web of Science | 14 |