| Yazarlar |
Dr. Öğr. Üyesi Nail Tekin ÖNDER
Kafkas Üniversitesi, Türkiye |
Zekariya Nur
Bursa Uludağ Üniversitesi, Türkiye |
|
Selim Alçay
Bursa Uludağ Üniversitesi, Türkiye |
| Özet |
| The aim of the present study was to evaluate the effects of an alpha-lipoic acid-supplemented extender on ram semen at a post-thaw stage and after incubation (6 hr). Semen samples were collected from five Kivircik Rams. Pooled semen was diluted with an egg yolk-based extender with different concentrations of alpha-lipoic acid (0.25 mmol L , 0.5 mmol L and 1 mmol L ) and without alpha-lipoic acid. Motility, plasma membrane functional integrity (HOST), acrosome integrity (FITC-Pisum sativum agglutinin) and DNA integrity (TUNEL) were assessed at post-thaw and 6 hr after incubation of the frozen-thawed semen. At the post-thaw stage, 0.25 mmol L alpha-lipoic acid had a positive effect on sperm motility and plasma membrane functional integrity compared to the control group (p < .05). At the post-incubation stage (6 hr), it was determined that the motility and plasma membrane functional integrity of the antioxidant groups were adversely affected compared to the control group (p < .05) and 1 mmol L dose of alpha-lipoic acid had a harmful effect on DNA integrity compared to the control group (p < .05). The results of the study demonstrated that alpha-lipoic acid has positive effects at post-thaw but have harmful effects on long term to ram semen. |
| Anahtar Kelimeler |
| alpha-lipoic acid | cryopreservation | incubation | ram semen |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | ANDROLOGIA |
| Dergi ISSN | 0303-4569 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 02-2022 |
| Cilt No | 54 |
| Sayı | 1 |
| Doi Numarası | 10.1111/and.14249 |
| Makale Linki | http://dx.doi.org/10.1111/and.14249 |
| Atıf Sayıları | |
| WoS | 9 |
