Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa
Yazarlar (8)
Prof. Dr. Selim Alçay Bursa Uludağ Üniversitesi, Türkiye
Arş. Gör. Elif Gökçe Kurt Tekirdağ Namık Kemal Üniversitesi, Türkiye
Mehmet Berk Toker
Doç. Dr. Nail Tekin ÖNDER Kafkas Üniversitesi, Türkiye
Prof. Dr. Burcu Üstüner Bursa Uludağ Üniversitesi, Türkiye
Doç. Dr. Ender Uzabacı Bursa Uludağ Üniversitesi, Türkiye
Doç. Dr. Zülfiye Gül Bahçeşehir Üniversitesi, Türkiye
Dr. Öğr. Üyesi Seda ÇAVUŞ ALAN Siirt Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | CRYOBIOLOGY (Q2) | ||
| Dergi ISSN | 0011-2240 Dergi Bilgileri (2016) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 06-2016 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-06-2016 |
| Cilt / Sayı / Sayfa | 72 / 3 / 269–273 | DOI | 10.1016/j.cryobiol.2016.03.007 |
| Makale Linki | http://dx.doi.org/10.1016/j.cryobiol.2016.03.007 | ||
| UAK Araştırma Alanları |
Dölerme ve Suni Tohumlama
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| Özet |
| The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine. |
| Anahtar Kelimeler |
| Freeze-drying | Goat semen | Cryopreservation | Antioxidants | Incubation resilience |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 38 |