| Yazarlar |
Selim Alçay
Bursa Uludağ Üniversitesi, Türkiye |
|
Elif Gökçe
Tekirdağ Namık Kemal Üniversitesi, Türkiye |
|
Mehmet Berk Toker
Türkiye |
Dr. Öğr. Üyesi Nail Tekin ÖNDER
Kafkas Üniversitesi, Türkiye |
|
Burcu Üstüner
Bursa Uludağ Üniversitesi, Türkiye |
|
Ender Uzabacı
Bursa Uludağ Üniversitesi, Türkiye |
|
Zülfiye Gül
Bahçeşehir Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Seda ÇAVUŞ ALAN
Siirt Üniversitesi, Türkiye |
| Özet |
| The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine. |
| Anahtar Kelimeler |
| Freeze-drying | Goat semen | Cryopreservation | Antioxidants | Incubation resilience |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | CRYOBIOLOGY |
| Dergi ISSN | 0011-2240 |
| Dergi Tarandığı Indeksler | SSCI |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 06-2016 |
| Cilt No | 72 |
| Sayı | 3 |
| Sayfalar | 269 / 273 |
| Doi Numarası | 10.1016/j.cryobiol.2016.03.007 |
| Makale Linki | http://dx.doi.org/10.1016/j.cryobiol.2016.03.007 |
| Atıf Sayıları | |
| WoS | 31 |
