| Yazarlar |
Selim Alçay
Bursa Uludağ Üniversitesi, Türkiye |
|
Mehmet Berk Toker
Türkiye |
|
Elif Gökçe
Tekirdağ Namık Kemal Üniversitesi, Türkiye |
|
Burcu Üstüner
Bursa Uludağ Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Nail Tekin ÖNDER
Kafkas Üniversitesi, Türkiye |
|
Hakan Sağırkaya
Bursa Uludağ Üniversitesi, Türkiye |
|
Zekariya Nur
Bursa Uludağ Üniversitesi, Türkiye |
|
Mustafa Kemal Soylu
Bursa Uludağ Üniversitesi, Türkiye |
| Özet |
| The aim of this study was to evaluate the effects of lyophilized egg yolk extender on ram semen cryopreservation. Ejaculates with a thick consistency, rapid wave motion (3-5 on a 0-5 scale) and >75% initial motility were pooled. Sperm were diluted to final concentration of 1/5 (semen/extender) in lyophilized egg yolk or fresh egg yolk extenders using two-step dilution method. The equilibrated semen was frozen in 0.25 mL straws. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) at three time points: after dilution with extender A, equilibration and post-thaw. The results showed that freezing and thawing procedures (dilution, equilibration and thawing) had negative effects on motility (P<0.001), plasma membrane integrity (P<0.001), acrosome integrity (P<0.001) and DNA integrity (P<0.001). In the study, there were no significant differences between lyophilized and fresh egg yolk extenders when comparing motility, plasma membrane integrity, acrosome integrity and DNA integrity between groups. In conclusion, lyophilized egg yolk extender provided similar cryoprotective effects with fresh egg yolk extender to cryopreserve ram semen. |
| Anahtar Kelimeler |
| Lyophilization,Egg yolk,Ram semen,Cryopreservation |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Cryobiology |
| Dergi ISSN | 0011-2240 |
| Dergi Tarandığı Indeksler | SSCI |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2015 |
| Cilt No | 71 |
| Sayfalar | 329 / 333 |
| Doi Numarası | 10.1016/j.cryobiol.2015.08.008 |
| Makale Linki | http://dx.doi.org/10.1016/j.cryobiol.2015.08.008 |
| Atıf Sayıları | |
| WoS | 40 |
