| Yazarlar |
Prof. Dr. Volkan YILMAZ
Kafkas Üniversitesi, Türkiye |
Yakup Yıldırım
Mehmet Akif Ersoy Üniversitesi, Türkiye |
|
Dr. Öğr. Üyesi Nüvit COŞKUN
Kafkas Üniversitesi, Türkiye |
| Özet |
| This study is a serological and virological examination of the border disease virus (BDV) in sheep at 1–5 years of age from private small scale production units of less than 20 sheep per unit, in the Kars District of Turkey. For this purpose, blood sera from 460 sheep were tested for antibodies against BDV using a commercial enzyme-linked immunosorbent assay (ELISA). Since BDV causes persistent infection, antigen-ELISA was also performed for this agent. Seropositivity rate was detected to be 74.57%. In addition, the BDV antigen was detected in one sample of seronegative sera (0.85%). Reverse transcription polymerase chain reaction (RTPCR) technique was used to determine the presence of pestivirus nucleic acid by using 5’UTR primer pair. Pestivirus nucleic acid was found in 2 of 117 seronegative samples (1.71%) by RTPCR. The results suggest that the infection was spreading in private small scale production units. Furthermore, recommendations for the control of BDV infection are presented. This study is the first molecular and serological study to determine viroprevalence and seroprevalence of BDV infection in sheep in the Kars District of Turkey. |
| Anahtar Kelimeler |
| BDV | ELISA | RT-PCR | Seroprevalence | Turkey |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Acta Veterinaria Brno |
| Dergi ISSN | 0001-7213 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 09-2014 |
| Cilt No | 83 |
| Sayı | 3 |
| Sayfalar | 175 / 180 |
| Doi Numarası | 10.2754/avb201483030175 |
| Atıf Sayıları | |
| SCOPUS | 7 |
| Google Scholar | 12 |
