Validated electrochemical immunosensor for ultra-sensitive procalcitonin detection: Carbon electrode modified with gold nanoparticles functionalized sulfur doped MXene as sensor platform and carboxylated graphitic carbon nitride as signal amplification

MEDETALİBEYOĞLU, HİLAL; BEYTUR, MURAT; AKYILDIRIM, ONUR; Atar, Necip; Yola, Mehmet Lütfi

doi:10.1016/j.snb.2020.128195

Validated electrochemical immunosensor for ultra-sensitive procalcitonin detection: Carbon electrode modified with gold nanoparticles functionalized sulfur doped MXene as sensor platform and carboxylated graphitic carbon nitride as signal amplification

Yazarlar (5)

Doç. Dr. Hilal MEDETALİBEYOĞLU Kafkas Üniversitesi, Türkiye

Doç. Dr. Murat BEYTUR Kafkas Üniversitesi, Türkiye

Doç. Dr. Onur AKYILDIRIM Kafkas Üniversitesi, Türkiye

Necip Atar Pamukkale Üniversitesi, Türkiye

Mehmet Lütfi Yola Hasan Kalyoncu Üniversitesi, Türkiye

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	Sensors and Actuators B Chemical (Q1)
Dergi ISSN	0925-4005 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI-Expanded
Makale Dili	İngilizce	Basım Tarihi	09-2020
Cilt / Sayı / Sayfa	319 / 1 / 128195–	DOI	10.1016/j.snb.2020.128195
Makale Linki	http://dx.doi.org/10.1016/j.snb.2020.128195

Özet

Septicemia, also known as sepsis, refers to a systemic inflammatory response syndrome and becomes the dominant reason of mortality for seriously diseases. Procalcitonin (PCT), the peptide precursor of the hormones, is a key biomarker of septicemia in the diagnosis and detection of bacterial inflammation. In this study, an ultra-sensitive sandwich type electrochemical immunosensor for PCT detection was constructed. Firstly, delaminated sulfur-doped MXene (d-S-Ti3C2TX MXene) modified glassy carbon electrode (GCE) including gold nanoparticles (AuNPs) was utilized as immunosensor platform to increase the amount of PCT antibody1 (Ab1). After that, carboxylated graphitic carbon nitride (c-g-C3N4) was used to label PCT Ab2 as signal amplification. The structure of electrochemical immunosensor was highlighted by x-ray diffraction (XRD) method, scanning electron microscope (SEM), transmission electron microscopy (TEM), x-ray photoelectron spectroscopy (XPS), fourier transform infrared spectroscopy (FTIR), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Herein, c-g-C3N4 not only has excellent catalytic activity toward H2O2 for signal amplification, but also can be directly utilized as redox probe. The analytical results have revealed that 0.01 - 1.0 pg mL^-1 and 2.0 fg mL^-1 were found as linearity range and limit of detection (LOD). Furthermore, the validated electrochemical immunosensor was examined in terms of stability, repeatability, reproducibility and reusability. Finally, the immunosensor was applied to plasma samples having high recovery.

Anahtar Kelimeler

Carboxylated graphitic carbon nitride | Immunosensor | Procalcitonin | Sulfur-doped MXene | Validation

Science Direct

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
Web of Science	111
Scopus	119

Validated electrochemical immunosensor for ultra-sensitive procalcitonin detection: Carbon electrode modified with gold nanoparticles functionalized sulfur doped MXene as sensor platform and carboxylated graphitic carbon nitride as signal amplification

Dergi Adı	SENSORS AND ACTUATORS B-CHEMICAL
Yayıncı	Elsevier B.V.
Açık Erişim	Hayır
E-ISSN	0925-4005
CiteScore	15,6
SJR	1,509
SNIP	1,332

Validated electrochemical immunosensor for ultra-sensitive procalcitonin detection: Carbon electrode modified with gold nanoparticles functionalized sulfur doped MXene as sensor platform and carboxylated graphitic carbon nitride as signal amplification

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