Evaluating the activity and stability of sonochemically produced hemoglobin-copper hybrid nanoflowers against some metallic ions, organic solvents, and inhibitors
 
Yazarlar (5)
Doç. Dr. Canan GÜLMEZ SAMSA Iğdır Üniversitesi, Türkiye
Doç. Dr. Cevahir Altınkaynak Nevşehir Hacı Bektaş Veli Üniversitesi, Türkiye
Melek Öztürkler
Prof. Dr. Nalan Özdemir Erciyes Üniversitesi, Türkiye
Prof. Dr. Onur ATAKİŞİ Kafkas Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Dergi ISSN 1389-1723 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 10-2021
Kabul Tarihi Yayınlanma Tarihi 01-10-2021
Cilt / Sayı / Sayfa 132 / 4 / 327–336 DOI 10.1016/j.jbiosc.2021.06.002
Makale Linki https://linkinghub.elsevier.com/retrieve/pii/S1389172321001481
UAK Araştırma Alanları
Veteriner Biyokimyası
Özet
The disadvantage of the conventional protein-inorganic hybrid nanoflower production method is the long incubation period of the synthesis method. This period is not suitable for practical industrial use. Herein, protein-inorganic hybrid nanoflowers were synthesized using hemoglobin and copper ion by fast sonication method for 10 min. The synthesized nanoflowers were characterized via scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and Fouirer-transform infrared spectroscopy. The activity and stability of the nanoflowers in the presence of different metal ions, organic solvents, inhibitors, and storage conditions were also evaluated by comparing with free hemoglobin. According to obtained results, the optimum pH and temperatures of both hybrid nanoflower and free hemoglobin were pH 5 and 40 °C, respectively. At all pH levels, nanoflower was more stable than free protein …
Anahtar Kelimeler
Protein-inorganic hybrid nanoflower | Sonication | Peroxidase mimic | Metal ions | Organic solvents | Inhibitors