Lipopolysaccharide treatment changes plasma total oxidant and antioxidant capacity on a time dependent manner in rabbits
Yazarlar (4)
Prof. Dr. Emine ATAKİŞİ Kafkas Üniversitesi, Türkiye
Prof. Dr. Onur ATAKİŞİ Kafkas Üniversitesi, Türkiye
Canan Gülmez
Doç. Dr. Kezban YILDIZ DALGINLI Kafkas Üniversitesi, Türkiye
| Bildiri Türü |
Tebliğ/Bildiri
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Bildiri Dili | İngilizce |
| Bildiri Alt Türü | Özet Metin Olarak Yayınlanan Tebliğ (Uluslararası Kongre/Sempozyum) | ||
| Bildiri Niteliği | Alanında Hakemli Uluslararası Kongre/Sempozyum | ||
| Kongre Adı | 3rd International Conference on New Trends in Chemistry | ||
| Kongre Tarihi | 28-04-2017 / 30-04-2017 | ||
| Basıldığı Ülke | Finlandiya | Basıldığı Şehir | Helsınkı |
| UAK Araştırma Alanları |
Veteriner Biyokimyası
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| Özet |
| It is well known that lipopolysaccharide (LPS), an outer-membrane component of gramnegative bacteria has frequently been used in experimental models of inflammation and oxidative stress [1-3]. Oxidative stress is described as an imbalance between reactive oxygen species (ROS) and scavenger systems (antioxidants). It can be caused by the inflammatory response to sepsis.[4]In this study, it was aimed to investigate the dose and time dependent effects of bacterial LPS on antioxidant status by evaluating total antioxidant capacity (TAC), total oxidant capacity (TOC)) and other biochemical parameters (total protein, albumin, globulin, albumin/globulin ratio in rabbits. Prior to LPS injection, blood samples were collected from 15 New Zealand rabbits (weighing 3-3.5 kg, at the age of 20-24 months) and these samples were named as 0th h. Then, 150 µg/kg LPS was intravenously injected through ear veins to the Group I (n= 7) and 300 µg/kg LPS to the Group II (n= 8). Following injections, plasma samples were separated after taking blood samples into heparinized pits at 0st, 1st, 2nd and 3rd h. Biochemical parameters were measured by colorimetrically using commercial kits. While TAC level was decreased 2nd and 3rd h, TOC level was increased in 3rd h in group I (p< 0.005 and p< 0.01 respectively) compared to the 0th h. Although TAC level was decreased in 1st, 2nd and 3rd h in group II, TOC level was increased in 2nd and 3rd h (p< 0.00, p< 0.05) compared to the 0th h. No significant difference was found in other biochemical parameters. In conclusion, while some blood biochemical parameters (total protein, albumin, globulin, albumin/globulin … |
| Anahtar Kelimeler |
