| Yazarlar |
Prof. Dr. Özkan ÖZDEN
Kafkas Üniversitesi, Türkiye |
Betty L Black
Türkiye |
|
Christopher M Ashwell
Türkiye |
|
Christian K Tipsmark
Türkiye |
|
Russell J Borski
Türkiye |
|
Brenda J Grubb
Türkiye |
| Özet |
| Proteins in the claudin family are a main component of tight junctions and form a seal that modulates paracellular transport in intestinal epithelium. This research tests the hypothesis that claudins 3, 5, and 16 will appear in the epithelium of embryonic intestine during functional differentiation. Immunohistochemistry is utilized to explore the developmental patterns of claudin-3, -5, and -16 proteins in the epithelium of embryonic chick intestine from 9 days prior to hatching through the early post-hatch period. These claudin proteins either changed their cellular localization or first appeared around the time of hatching. After hatching, claudin-3 expression was prominent in basal-lateral regions of the epithelium along the entire villus, but was absent from crypts. Claudin-5 was expressed most strongly in the crypt and lower villus epithelium within junctional complexes, whereas immunostaining of claudin-16 was localized within goblet cells of the upper villus region. The relative mRNA levels of claudin-3, -5, and -16 showed similar patterns; transcript levels rose between 18 and 20 days of development, then dropped by 2 days post-hatch. Results of this work indicate that the claudin proteins assume their final locations within the epithelium around the time of hatching, suggesting that in addition to their known barrier and fence functions within tight junctions, these claudins may have additional roles in the differentiation and/or physiological function of chick intestine. The localization of claudin-16 to goblet cells and its distribution in the more mature cells of the upper villus region suggest an unexpected role in goblet cell maturation and mucus secretion. |
| Anahtar Kelimeler |
| claudin proteins | tight junctions | intestine | goblet cells | chick embryo |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY |
| Dergi ISSN | 1932-8486 |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 07-2010 |
| Cilt No | 293 |
| Sayı | 7 |
| Sayfalar | 1175 / 1183 |
| Doi Numarası | 10.1002/ar.21163 |
| Makale Linki | http://doi.wiley.com/10.1002/ar.21163 |
| Atıf Sayıları | |
| WoS | 29 |
