| Yazarlar |
Sibel Can
Türkiye |
|
Jale Selli
Atatürk Üniversitesi, Türkiye |
|
Başak Büyük
Atatürk Üniversitesi, Türkiye |
|
Sergülen Aydın
Kafkas Üniversitesi, Türkiye |
Prof. Dr. Ramazan KOCAASLAN
Kafkas Üniversitesi, Türkiye |
|
Güvendi Gf
Türkiye |
| Özet |
| Recent years, lots of scientific studies are focused on the possible mechanism of inflammatory response and oxidative stress which are the mechanism related with tissue damage and exercise fatigue. It is well-known that free oxygen radicals may be induced under invitro conditions as well as oxidative stress by exhaustive physical exercise. The aim of this study was to investigate the effects of anabolic steroids in conjunction with exercise in the process of spermatogenesis in the testes, using histological and stereological methods. Thirty-six male Sprague Dawley rats were divided to six groups, including the control group, the eccentric exercise administered group, the estrogen applied group, the estrogen applied and dissected one hour after eccentric exercise group, the no estrogen applied and dissected 48 hours after eccentric exercise group and the estrogen applied and dissected 48 hours after eccentric exercise group. Eccentric exercise was performed on a motorized rodent treadmill and the estrogen applied groups received daily physiological doses by subcutaneous injections. Testicular tissues were examined using specific histopathological, immunohistochemical and stereological methods. Sections of the testes tissue were stained using the TUNEL method to identify apoptotic cells. Apoptosis was calculated as the percentage of positive cells, using stereological analysis. A statistical analysis of the data was carried out with one-way analysis of variance (ANOVA) for the data obtained from stereological analysis. Conventional light microscopic results revealed that testes tissues of the eccentric exercise administered group and the estrogen supplemented group exhibited slight impairment. In groups that were both eccentrically exercised and estrogen supplemented, more deterioration was detected in testes tissues. Likewise, immunohistochemistry findings were also more prominent in the eccentrically exercised and estrogen supplemented groups. The findings suggest that estrogen supplementation increases damage in testicular tissue due to eccentric exercise. |
| Anahtar Kelimeler |
| Exercise | Testis | Estrogens | Rats |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | IRANIAN RED CRESCENT MEDICAL JOURNAL |
| Dergi ISSN | 2074-1804 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 04-2015 |
| Cilt No | 17 |
| Sayı | 4 |
| Doi Numarası | 10.5812/ircmj.17(4)2015.22521 |
| Makale Linki | http://www.ircmj.com/?page=article&article_id=22521 |
| Atıf Sayıları | |
| Google Scholar | 4 |
