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Association of luteal blood flow with follicular size serum estrogen and progesterone concentrations and the inducibility of luteolysis by PGF2 in dairy cows    
Yazarlar
Prof. Dr. Semra KAYA Prof. Dr. Semra KAYA
Kafkas Üniversitesi, Türkiye
Prof. Dr. Cihan KAÇAR Prof. Dr. Cihan KAÇAR
Kafkas Üniversitesi, Türkiye
Bülent Polat
Türkiye
Armağan Çolak
Atatürk Üniversitesi, Türkiye
Duygu Kaya
Kafkas Üniversitesi, Türkiye
İsmayil Safa Gürcan
Ankara Üniversitesi, Türkiye
Bollweın Heınrıch
Türkiye
Selim Aslan
Yakın Doğu Üniversitesi, Türkiye
Özet
The aim of this study was to investigate the compatibility of the visual evaluation result of the blood flow characteristics and the blood flow measurements of the CL and the predictability of the responses given by corpora lutea with varying levels of blood flow to an induction of luteolysis by a PGF injection and to determine the possibility of increase in serum estrogen and progesterone concentrations in parallel with increased luteal blood flow (LBF). The cows, bearing a CL (n = 60; postpartum 35 days), were injected with PGF and were monitored for signs of estrous following the first injection. The cows, which did not show estrous signs, were examined for the presence of a CL on Day 14, whereas those that showed signs of estrous were examined on Day 10 following the onset of estrous. The level of LBF was visually graded as + (low; GI), ++ (medium; GII), +++ (high; GIII), and ++++ (very high; GIV). Immediately after the examination of LBFs, a second intramuscular injection of PGF was injected. In the cows, which were determined to be in estrous, the diameter of the Graafian follicles was measured by B-mode ultrasonography. Subsequently, these animals were artificially inseminated. The animals, which did not show estrous after the second injection, were examined as previously described and monitored for signs of estrous. A strong correlation (r = 0.654; P < 0.001) was determined to exist between the results of the visual examination of the images and the results obtained for the LBF area with the use of the Pixel Flux software. GIII (0.83 ± 0.15 cm) and GIV (1.03 ± 0.48 cm) were found to differ from GI (0.47 ± 0.23 cm) and GII (0.51 ± 0.12 cm) for the size of the LBF (P < 0.001). Serum progesterone levels in groups (GI, GII, GIII, and GIV) were determined to be 4.44 ± 2.42 ng/mL, 6.03 ± 2.37 ng/mL, 7.01 ± 2.94 ng/mL, and 7.17 ± 1.69 ng/mL, respectively. The comparative evaluation of the study groups showed that the groups did not statistically differ for the period between PGF injection and the onset of estrous, mean Graafian follicle size and estrogen levels. No direct correlation existed between these reproductive parameters and LBF.
Anahtar Kelimeler
Cow | Power Doppler ultrasonography | Corpus luteum | PGF(2 alpha)
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı THERIOGENOLOGY
Dergi ISSN 0093-691X
Dergi Tarandığı Indeksler SSCI
Dergi Grubu Q1
Makale Dili İngilizce
Basım Tarihi 01-2017
Cilt No 87
Sayı 1
Sayfalar 167 / 172
Doi Numarası 10.1016/j.theriogenology.2016.08.022
Makale Linki http://linkinghub.elsevier.com/retrieve/pii/S0093691X16303879