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Efficacy of a commercial test kit to determine early pregnancy in cows using whole blood and blood serum    
Yazarlar
Ozan Karakuş
Türkiye
Prof. Dr. Cihan KAÇAR
Kafkas Üniversitesi, Türkiye
Doç. Dr. Mushap KURU
Kafkas Üniversitesi, Türkiye
Prof. Dr. Semra KAYA
Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Murat Can DEMİR
Kafkas Üniversitesi, Türkiye
Prof. Dr. Umut Çağın ARI
Kafkas Üniversitesi, Türkiye
Abuzer Kafar Zonturlu
Harran Üniversitesi, Türkiye
Prof. Dr. Yavuz ÖZTÜRKLER
Kafkas Üniversitesi, Türkiye
Özet
The objective of this study was to determine the efficacy of the Fassisi®BoviPreg visual test kit (Fassisi®BoviPreg) in determining early pregnancies in cows by measuring the pregnancy-associated glycoprotein (PAG) in whole blood and blood serum. The study was conducted on 50 cows, the artificial insemination (AI) dates of which were designated as day 0. Pregnancy diagnosis was performed with transrectal ultrasonography (USG), and serum samples were simultaneously collected and used with Fassisi®BoviPreg to determine pregnancies on 30 days after AI. The results of the Fassisi®BoviPreg test on serum and whole blood samples, respectively, on 30 days after AI were as follows: sensitivity, 61.54% and 50.0%; specificity, 79.17% and 75%; accuracy, 70.0% and 62.0%; positive predictive values, 76.2% and 68.4%; negative predictive values, 65.5% and 58.1%; false-positive diagnoses, 23.8% and 31.6%; and false-negative diagnoses, 34.5% and 41.9%. On day 50 after AI, sensitivities were 63.64% and 50.0%, specificities were 100.0% and 100.0%, and accuracies were 75.0% and 65.62% in serum and whole blood, respectively. Higher pregnancy rates were obtained using Fassisi®BoviPreg in cows with ≥ 5 ng/mL P4 (P < 0.001). In conclusion, the results from the Fassisi®BoviPreg tests on cows on 30 and 50 days after AI showed that the use of blood serum is more accurate and suitable than that of whole blood. The results also showed a higher confidence level in specificity 50 days after AI.
Anahtar Kelimeler
Cow,Early pregnancy,PAG,Progesterone
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı TROPICAL ANIMAL HEALTH AND PRODUCTION
Dergi ISSN 0049-4747
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q2
Makale Dili İngilizce
Basım Tarihi 01-2021
Cilt No 53
Sayı 28
Sayfalar 1 / 6
Doi Numarası 10.1007/s11250-020-02428-x