| Yazarlar |
Eliş Yıldız S
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|
Nazlı M
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|
Nur G
|
| Özet |
| Aim: In this study, the effects of capsaicin on the immunohistochemical distribution and gene expression of transforming growth factor alpha (TGF-α) in ovarian tissue of rats at the pubertal age were examined. Materials and methods: Rats (50 days old) were divided into experimental, alimentation, and sham groups. The experimental group was injected with 1 mg/kg doses of capsaicin (10% ethanol and 1% Tween in an 80% distilled water mixture) subcutaneously every day for a period of 1 week. The alimentation group received 1 mg/kg doses of capsaicin (10% ethanol and 1% Tween in an 80% distilled water mixture). The sham group was injected with a solution of 10% ethanol and 1% Tween in a mixture of 80% distilled water. Body weights and ovary weights were compared within the groups. Triple staining methods were used for the histological examinations. The tissue localization of TGF-α was analyzed using an immunohistochemical method and gene expression level was determined using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Results: No difference in the structure of the 3 groups was observed histologically. Immunohistochemically, TGF-α immunoreactivity was detected in the primordial follicles, primary follicles, theca cells, and interstitial cells at a medium density in all groups. The effect of capsaicin on TGF-α with mRNA was not found to be significantly different among the 3 groups. Conclusion: The results showed that capsaicin had no effects on ovaries at different developmental stages. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Turkish Journal of Medical Sciences |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 02-2013 |
| Cilt No | 43 |
| Sayı | 2 |
| Sayfalar | 326 / 332 |
