Anti microbial and Anti oxidant Activity of Watermelon (Citrulluslanatus) Fruit and Watermelon Seed
  
Yazarlar (4)
Perihan Akbaş Kafkas Üniversitesi, Türkiye
Özlem Gürsoy Kol Kafkas Üniversitesi, Türkiye
Abdurrahman Gürbüz
Kafkas Üniversitesi, Türkiye
Doç. Dr. Sevda MANAP Kafkas Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayınlanan tam makale)
Dergi Adı Celal Bayar Üniversitesi Fen Bilimleri Dergisi
Dergi ISSN 1305-130X
Dergi Tarandığı Indeksler TR DİZİN
Makale Dili İngilizce Basım Tarihi 03-2017
Cilt / Sayı / Sayfa 13 / 1 / 139–147 DOI 10.18466/cbujos.302659
Özet
Watermelon (Citrullus lanatus) flesh and seeds were dried and pulverized, and their extracts were diffused to sterilediscs for the evaluation of anti-microbial activity. The disc-diffusion technique was used to assess anti-bacterial activityagainst Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Pasteurella multocida, Yersinia enterocolitica, Serratiamarcescens, Klebsiella pneumoniae, Xanthomonas campestris, Staphylococcus aureus. Anti-fungal activity against the yeastsCandida albicans and Rhodotorula glutinis was also examined. Standard anti-biotics were also tested as controls.Watermelon flesh and seed extracts were found to be effective against gram-positive and gram-negative bacteria andyeasts. The extracts were also screened for anti-oxidant activity using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) freeradical-scavenging assay, total reducing ability using the Fe3+–Fe2+ transformation method and ferrous ion (Fe2+)-chelating activity. Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), ethylenediaminetetraacetic acidand α-tocopherol were used as reference anti-oxidant radical scavenger compounds.The most potent anti-bacterial activity was demonstrated by watermelon–ethanol extract (inhibition zone 30 mm)against K. pneumoniae, and the most potent anti-fungal activity was demonstrated by watermelon–acetone extract(inhibition zone 26 mm) against R. glutinis. Watermelon–ethanol and watermelon seed–ethanol extracts both demonstratedmarked anti-oxidant activity.These results highlight that watermelon fruit and seed extracts have potential for the development of efficient, safeand cost-effective natural anti-oxidant compounds for application in the functional food industries.
Anahtar Kelimeler