GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum
Yazarlar (2)
Prof. Dr. Serap KORAL TAŞÇI Kafkas Üniversitesi, Türkiye
Prof. Dr. Seyit Ali BİNGÖL Kafkas Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Journal of Veterinary Research | ||
| Dergi ISSN | 2450-7393 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 06-2018 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-06-2018 |
| Cilt / Sayı / Sayfa | 62 / 2 / 237–242 | DOI | 10.2478/jvetres-2018-0033 |
| Makale Linki | https://content.sciendo.com/view/journals/jvetres/62/2/article-p237.xml | ||
| UAK Araştırma Alanları |
Histoloji ve Embriyoloji
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| Özet |
| Abstract Introduction Glucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction. Material and Methods This study included 18 male Balb/c mice that were divided into diabetic, sham, and control groups. Mice in the diabetic group received 100 mg/kg of streptozotocin. Immunohistochemical expression of GLP-1 was determined using the avidin–biotin–peroxidase complex technique, and proglucagon gene expression was determined by RT-PCR. Results Analysis of GLP-1 immunohistochemical localisation showed that GLP-1-immunopositive cells (L cells) were present between epithelial cells in the intestinal crypts. The intensity and localisation of GLP-1 immunoreactivity were similar among the mice in all the groups. Proglucagon gene expression levels were also statistically similar among the mice in all the groups. Conclusion No difference was demonstrated among the mice in the diabetic, sham, or control groups with respect to proglucagon gene expression and GLP-1 localisation in the ileum, suggesting that diabetes does not affect proglucagon gene expression in the ileum. |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |