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Phylogenetic analysis of West Nile virus: first report of lineage 1 in donkey in Turkey    
Yazarlar
Yakup Yıldırım
Burdur Mehmet Akif Ersoy Üniversitesi, Türkiye
Prof. Dr. Volkan YILMAZ Prof. Dr. Volkan YILMAZ
Kafkas Üniversitesi, Türkiye
Kemal Yazıcı
Ardahan Üniversitesi, Türkiye
Dr. Öğr. Üyesi Cem ÖZİÇ Dr. Öğr. Üyesi Cem ÖZİÇ
Kafkas Üniversitesi, Türkiye
Aykut Özkul
Ankara Üniversitesi, Türkiye
Abdurrahman Anıl Çağırgan
Türkiye
Özet
West Nile virus (WNV), a member of the Flaviviridae, is a major arbovirus that causes West Nile fever. Previous data showed the prevalence of the WNV serologically and molecular in Turkey, and the presence of lineage 1 in horses and humans has been reported. This is the first notification of partial phylogeny of WNV detected in donkeys in the northeast of Turkey (on the Iranian border). Blood serum samples collected from 25 donkeys without clinical symptoms were tested by RT-PCR. Sequence analysis of the sample detected as positive was performed. Multiple sequence alignments of reference sequences taken from GenBank were performed using the ClustalW method using the MEGA6 program. Partial nucleotide sequences of the capsid gene coding region revealed that the strains are closely related to viruses of lineage 1, clade 1a. According to the phylogenetic tree, the TUR/Igdir/donkey strain was included in the same cluster as the strain (KJ958922) previously obtained from horses in Turkey and the strain (GQ851658) from the Central African Republic. This study is the first report to show the circulation of WNV lineage 1 in donkeys in Turkey.
Anahtar Kelimeler
Donkey | Northeast Anatolia | Phylogeny | Turkey | West Nile virus
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı TROPICAL ANIMAL HEALTH AND PRODUCTION
Dergi ISSN 0049-4747
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q2
Makale Dili İngilizce
Basım Tarihi 11-2021
Cilt No 53
Sayı 5
Sayfalar 3 / 6
Doi Numarası 10.1007/s11250-021-02892-z
Makale Linki http://dx.doi.org/10.1007/s11250-021-02892-z