Engineered P450 BM3 and cpADH5 coupled cascade reaction for β-oxo fatty acid methyl ester production in whole cells
Yazarlar (4)
Dr. Öğr. Üyesi Yunus ENSARİ Kafkas Üniversitesi, Türkiye
Gustavo De Almeida Santos Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
Anna Joelle Ruff Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
Ulrich Schwaneberg Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Enzyme and Microbial Technology | ||
| Dergi ISSN | 0141-0229 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 08-2020 |
| Cilt / Sayı / Sayfa | 138 / 1 / 109555–0 | DOI | 10.1016/j.enzmictec.2020.109555 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S014102292030048X | ||
| Özet |
| Hydroxy- or ketone- functionalized fatty acid methyl esters (FAMEs) are important compounds for production of pharmaceuticals, vitamins, cosmetics or dietary supplements. Biocatalysis through enzymatic cascades has drawn attention to the efficient, sustainable, and greener synthetic processes. Furthermore, whole cell catalysts offer important advantages such as cofactor regeneration by cell metabolism, omission of protein purification steps and increased enzyme stability. Here, we report the first whole cell catalysis employing an engineered P450 BM3 variant and cpADH5 coupled cascade reaction for the biosynthesis of hydroxy- and keto-FAMEs. Firstly, P450 BM3 was engineered through the KnowVolution approach yielding P450 BM3 variant YE_M1_2, (R47S/Y51W/T235S/N239R/I401 M) which exhibited boosted performance toward methyl hexanoate. The initial oxidation rate of YE_M1_2 toward methyl … |
| Anahtar Kelimeler |
| directed evolution | P450 BM3 | Alcohol dehydrogenase | whole cell | biocatalysis | fatty acid methyl ester |
Science Direct
Engineered P450 BM3 and cpADH5 coupled cascade reaction for β-oxo fatty acid methyl ester production in whole cells
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 16 |
| Web of Science | 10 |