A Comparative Reengineering Study of cpADH5 through Iterative and Simultaneous Multisite Saturation Mutagenesis
Yazarlar (5)
Dr. Öğr. Üyesi Yunus ENSARİ Kafkas Üniversitesi, Türkiye
Gaurao V. Dhoke Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
Mehdi D. Davari Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
Anna Joëlle Ruff Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
Ulrich Schwaneberg Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Chembiochem (Q1) | ||
| Dergi ISSN | 1439-4227 Dergi Bilgileri (2018) | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 07-2018 |
| Cilt / Sayı / Sayfa | 19 / 14 / 1563–1569 | DOI | 10.1002/cbic.201800159 |
| Makale Linki | http://doi.wiley.com/10.1002/cbic.201800159 | ||
| UAK Araştırma Alanları |
Biyoteknoloji
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| Özet |
| Positions identified in directed evolution campaigns or by (semi)rational design can be recombined iteratively or simultaneously. Iterative recombination has yielded many success stories and is beneficially used if screening capabilities are limited (four iterative SSMs generate 20×4=80 different enzyme variants). Simultaneous site saturation mutagenesis offers significantly higher diversity (204=160 000 variants) and enables greater improvements to be found, especially if the selected positions are in close proximity to each other (cooperative effects). Here we report a first comprehensive comparison of iterative and simultaneous saturation of four residues in Candida parapsilosis alcohol dehydrogenase 5 (cpADH5) with methyl 3‐hydroxyhexanoate as substrate. Screening of 7200 clones from 33 site saturation mutagenesis libraries (exploring 17 recombination paths) yielded the cpADH5 W286A variant, with a … |
| Anahtar Kelimeler |
| Directed evolution | OmniChange | Protein engineering | Recombination | Simultaneous saturation |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 11 |
| Scopus | 13 |
| Google Scholar | 18 |