Inversion of cpADH5 Enantiopreference and Altered Chain Length Specificity for Methyl 3-Hydroxyalkanoates
Yazarlar (6)
Dr. Öğr. Üyesi Yunus ENSARİ Kafkas Üniversitesi, Türkiye
Gaurao V. Dhoke Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
Mehdi D. Davari Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
Marco Bocola Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
Anna Joëlle Ruff Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
Ulrich Schwaneberg Rheinisch-Westfälische Technische Hochschule Aachen, Almanya
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Chemistry A European Journal | ||
| Dergi ISSN | 0947-6539 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 09-2017 |
| Cilt / Sayı / Sayfa | 23 / 51 / 12636–12645 | DOI | 10.1002/chem.201702581 |
| Özet |
| Expanding the substrate scope of enzymes opens up new routes for synthesis of valuable chemicals. Ketone‐functionalized fatty acid derivatives and corresponding chiral alcohols are valuable building blocks for the synthesis of a variety of chemicals including pharmaceuticals. The alcohol dehydrogenase from Candida parapsilosis (cpADH5) catalyzes the reversible oxidations of chiral alcohols and has a broad substrate range; a challenge for cpADH5 is to convert alcohols with small substituents (methyl or ethyl) next to the oxidized alcohol moiety. Molecular docking studies revealed that W286 is located in the small binding pocket and limits the access to substrates that contain aliphatic chains longer than ethyl substituent. In the current manuscript, we report that positions L119 and W286 are key residues to boost oxidation of medium chain methyl 3‐hydroxy fatty acids; interestingly the enantiopreference toward … |
| Anahtar Kelimeler |
| alcohol dehydrogenase | directed evolution | fatty acid methyl ester | methyl 3-hydroxyalkanoate | protein engineering |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 7 |
| Web of Science | 5 |
| Scopus | 5 |