A novel double-antigen sandwich ELISA for the species-independent detection of Crimean-Congo hemorrhagic fever virus-specific antibodies
Yazarlar (8)
Miriam A. Sas
Friedrich-Loeffler-Institute, Almanya
Friedrich-Loeffler-Institute, Almanya
Loic Comtet
Kafkas Üniversitesi, Fransa
Kafkas Üniversitesi, Fransa
Fabien Donnet
Institut Pasteur, Paris, Fransa
Institut Pasteur, Paris, Fransa
Marc Mertens
Id Vet, Almanya
Id Vet, Almanya
Prof. Dr. Zati VATANSEVER Kafkas Üniversitesi, Türkiye
Noel Tordo Institut Pasteur, Paris, Fransa
Philippe Pourquier
Id Vet, Fransa
Id Vet, Fransa
Martin H Groschup
Friedrich-Loeffler-Institute, Almanya
Friedrich-Loeffler-Institute, Almanya
| Makale Türü | Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Antiviral Research | ||
| Dergi ISSN | 0166-3542 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Exp, SCOPUS, Biological Abstracts, Biosis Full Coverage Shared, Biosis Previews, Curation, Current Contents Life Sciences, Derwent Drug File, Essential Science Indicators, Pdf2xml, Pdf2xml, Prous, Reference Master, Sophia | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2018 |
| Cilt / Sayı / Sayfa | 151 / 1 / 24–26 | DOI | 10.1016/j.antiviral.2018.01.006 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S0166354217308124 | ||
| UAK Araştırma Alanları |
Veteriner Parazitolojisi
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| Özet |
| Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease in humans caused by the CCHF virus (CCHFV). The detection of anti-CCHFV antibodies in animals is used to reveal infection risk areas. Therefore a simple, quick and reliable multispecies assay for the detection of CCHFV-specific antibodies is needed. This work presents the development and validation of a novel CCHF double-antigen ELISA for the detection of anti-CCHFV nucleoprotein antibodies. The test requires 30 μl of serum, and results are obtained within 90 min. As the ELISA is based on recombinant N-protein of the IbAr10200 virus, it can be run under standard biosafety conditions. For assay validation, sera from 95 cattle and 176 small ruminants from CCHF-endemic regions (origin: Albania, Cameroon, Kosovo, Former Yugoslav Republic of Macedonia, Mauritania, Pakistan, Turkey) served as a positive reference serum panel. The ... |
| Anahtar Kelimeler |
| Crimean-Congo hemorrhagic fever virus | Double-antigen ELISA | Multi-species ELISA | Nairovirus | Tick-borne diseases |
Science Direct
A novel double-antigen sandwich ELISA for the species-independent detection of Crimean-Congo hemorrhagic fever virus-specific antibodies
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 84 |
| Scopus | 88 |
| Google Scholar | 121 |