A novel double-antigen sandwich ELISA for the species-independent detection of Crimean-Congo hemorrhagic fever virus-specific antibodies
Yazarlar (5)
Marc Mertens Friedrich-Loeffler-Institute, Almanya
Prof. Dr. Zati VATANSEVER Kafkas Üniversitesi, Türkiye
Noel Tordo
Institut Pasteur, Paris, Fransa
Institut Pasteur, Paris, Fransa
Philippe Pourquier
Id Vet, Fransa
Id Vet, Fransa
Martin H Groschup Friedrich-Loeffler-Institute, Almanya
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Antiviral Research (Q1) | ||
| Dergi ISSN | 0166-3542 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2018 |
| Cilt / Sayı / Sayfa | 151 / 1 / 24–26 | DOI | 10.1016/j.antiviral.2018.01.006 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S0166354217308124 | ||
| Özet |
| Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease in humans caused by the CCHF virus (CCHFV). The detection of anti-CCHFV antibodies in animals is used to reveal infection risk areas. Therefore a simple, quick and reliable multispecies assay for the detection of CCHFV-specific antibodies is needed. This work presents the development and validation of a novel CCHF double-antigen ELISA for the detection of anti-CCHFV nucleoprotein antibodies. The test requires 30 μl of serum, and results are obtained within 90 min. As the ELISA is based on recombinant N-protein of the IbAr10200 virus, it can be run under standard biosafety conditions. For assay validation, sera from 95 cattle and 176 small ruminants from CCHF-endemic regions (origin: Albania, Cameroon, Kosovo, Former Yugoslav Republic of Macedonia, Mauritania, Pakistan, Turkey) served as a positive reference serum panel. The … |
| Anahtar Kelimeler |
| Crimean-Congo hemorrhagic fever virus | Tick-borne diseases | Nairovirus | Double-antigen ELISA | Multi-species ELISA |
Science Direct
A novel double-antigen sandwich ELISA for the species-independent detection of Crimean-Congo hemorrhagic fever virus-specific antibodies
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 80 |
| SCOPUS | 85 |
| Google Scholar | 112 |