Circulation of Crimean Congo Hemorrhagic Fever Virus in the Former Yugoslav Republic of Macedonia Revealed by Screening of Cattle Sera Using a Novel Enzyme linked Immunosorbent Assay
Yazarlar (16)
Marc Mertens
Friedrich-Loeffler-Institute, Almanya
Prof. Dr. Zati VATANSEVER
Kafkas Üniversitesi, Türkiye
Slavcho Mrenoshki
Ss. Cyril And Methodius University İn Skopje, Kuzey Makedonya
Kiril Krstevski
Ss. Cyril And Methodius University İn Skopje, Kuzey Makedonya
Jovana Stefanovska
Ss. Cyril And Methodius University İn Skopje, Kuzey Makedonya
Igor Djadjovski
Ss. Cyril And Methodius University İn Skopje, Kuzey Makedonya
Iskra Cvetkovikj
Ss. Cyril And Methodius University İn Skopje, Kuzey Makedonya
Robert Farkas
Hungarian University Of Agriculture And Life Sciences, Macaristan
Isolde Schuster
Friedrich-Loeffler-Institute, Almanya
Fabien Donnet
Loic Comtet
Noël Tordo
Institut Pasteur, Paris, Fransa
Mohamed Ben Mechlia
Institut Pasteur, Paris, Fransa
Anne Balkema-Buschmann
Friedrich-Loeffler-Institute, Almanya
Dine Mitrov
Ss. Cyril And Methodius University İn Skopje, Kuzey Makedonya
Martin H. Groschup
Friedrich-Loeffler-Institute, Almanya
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Plos Neglected Tropical Diseases (Q1) | ||
| Dergi ISSN | 1935-2727 | ||
| Dergi Tarandığı Indeksler | SCI-Exp, SCOPUS, Biological Abstracts, Biosis Previews, Biosis Selective Coverage Shared, Curation, Current Contents Clinical Medicine, Derwent Drug File, Essential Science Indicators, Reference Master, Sophia, Zoological Record | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2015 |
| Cilt / Sayı / Sayfa | 9 / 3 / 3519–0 | DOI | 10.1371/journal.pntd.0003519 |
| Makale Linki | http://dx.plos.org/10.1371/journal.pntd.0003519 | ||
| Özet |
| There are only few assays available for the detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)-specific antibodies in animals, and data about diagnostic sensitivity and specificity are incompletely documented for most of these tests. This is unfortunate since CCHFV antibodies in animals can be used as indicator for virus circulation in a geographic area and therewith potential risk of human exposure. This paper therefore reports on a novel ELISA for the detection of CCHFV-specific antibodies in cattle and on its application for testing ruminant sera from the Former Yugoslav Republic of Macedonia. A highly sensitive and specific ELISA was developed to detect CCHFV-specific IgG antibodies in cattle. The assay was validated by using 503 negative serum samples from a country where CCHFV has never been detected until now, and by using 54 positive serum samples. The positive sera were verified by using two commercially available assays (for testing human serum) which we have adapted for use in animals. The sensitivity of the novel ELISA was 98% and its specificity 99%. The presence of Hyalomma ticks was demonstrated in the Former Yugoslav Republic of Macedonia and depending on the region antibody prevalence rates up to 80% were detected in the cattle population. This article describes a fully validated, highly sensitive and specific ELISA for the detection of CCHFV-specific IgG antibodies in cattle. Using this assay, CCHFV-specific antibodies were detected for the first time in cattle in the Former Yugoslav Republic of Macedonia, giving evidence for an active circulation of this virus in the country. Supporting this conclusion, the occurrence of the main vector of CCHFV was demonstrated in the present work for the first time in Former Yugoslav Republic of Macedonia. |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 44 |
| SCOPUS | 46 |
| Google Scholar | 64 |