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Molecular and serological evidence of Anaplasma phagocytophilum infection of farm animals in the Black Sea Region of Turkey        
Yazarlar
Halil İbrahim Gökçe
Türkiye
Oktay Genç
Türkiye
Atila Akca
Türkiye
Prof. Dr. Zati VATANSEVER Prof. Dr. Zati VATANSEVER
Ankara Üniversitesi, Türkiye
Ahmet Unver
Türkiye
Hidayet Metin Erdogan
Türkiye
Özet
This study was designed to determine the presence and the prevalence of Anaplasma phagocytophilum infection in sheep and cattle in the Middle and Eastern Black Sea Regions of Turkey in which the potential vector, Ixodes ricinus, is widespread. Blood samples were collected from 720 sheep and 720 cattle from 6 provinces of the region, and used for detecting antibodies to A. phagocytophilum by indirect immunofluorescent antibody test (IFAT) and specific nucleic acids by a nested polymerase chain reaction (PCR). Blood smears were also prepared and examined microscopically for the presence of A. phagocytophilum-like organisms in polymorphonuclear cells. Of the animals examined, antibodies were detected in 110 (15.27%) cattle and 107 (14.86%) sheep and A. phagocytophilum-like organisms were detected in the blood of 73 (10.13%) cattle and 71 (9.86%) sheep. In addition, specific DNA was detected in the blood of 27 (14.75%) cattle and 22 (12.35%) sheep. The results obtained constitute the first molecular and serological evidence of A. phagocytophilum infection in sheep and cattle in the Black Sea Region of Turkey. © 2008 Akadémiai Kiadó.
Anahtar Kelimeler
cattle | sheep | Anaplasma phagocylophilum | prevalence | seroprevalence | indirect immunofluorescent antibody test (IFAT) | polymerase chain reaction (PCR)
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı ACTA VETERINARIA HUNGARICA
Dergi ISSN 0236-6290
Dergi Tarandığı Indeksler SCI-Exp, SCOPUS, Biological Abstracts, Biosis Full Coverage Shared, Biosis Previews, Curation, Current Contents Agriculture Biology & Environmental Sciences, Essential Science Indicators, Pdf2xml, Pdf2xml, Reference Master, Sophia, Zoological Record
Dergi Grubu Q3
Makale Dili İngilizce
Basım Tarihi 09-2008
Cilt No 56
Sayı 3
Sayfalar 281 / 292
Doi Numarası 10.1556/AVet.56.2008.3.2
Makale Linki http://www.akademiai.com/doi/abs/10.1556/AVet.56.2008.3.2