Molecular and serological evidence of Anaplasma phagocytophilum infection of farm animals in the Black Sea Region of Turkey
       
Yazarlar (6)
Halil İbrahim Gökçe Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Oktay Genç Ondokuz Mayis Üniversitesi, Türkiye
Atila Akca Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Prof. Dr. Zati VATANSEVER Kafkas Üniversitesi, Türkiye
Ahmet Unver Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Hidayet Metin Erdogan Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Acta Veterinaria Hungarica (Q3)
Dergi ISSN 0236-6290 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Exp, SCOPUS, Biological Abstracts, Biosis Full Coverage Shared, Biosis Previews, Curation, Current Contents Agriculture Biology & Environmental Sciences, Essential Science Indicators, Pdf2xml, Pdf2xml, Reference Master, Sophia, Zoological Record
Makale Dili İngilizce Basım Tarihi 09-2008
Cilt / Sayı / Sayfa 56 / 3 / 281–292 DOI 10.1556/AVet.56.2008.3.2
Makale Linki http://www.akademiai.com/doi/abs/10.1556/AVet.56.2008.3.2
Özet
This study was designed to determine the presence and the prevalence of Anaplasma phagocytophilum infection in sheep and cattle in the Middle and Eastern Black Sea Regions of Turkey in which the potential vector, Ixodes ricinus, is widespread. Blood samples were collected from 720 sheep and 720 cattle from 6 provinces of the region, and used for detecting antibodies to A. phagocytophilum by indirect immunofluorescent antibody test (IFAT) and specific nucleic acids by a nested polymerase chain reaction (PCR). Blood smears were also prepared and examined microscopically for the presence of A. phagocytophilum-like organisms in polymorphonuclear cells. Of the animals examined, antibodies were detected in 110 (15.27%) cattle and 107 (14.86%) sheep and A. phagocytophilum-like organisms were detected in the blood of 73 (10.13%) cattle and 71 (9.86%) sheep. In addition, specific DNA was detected in the blood of 27 (14.75%) cattle and 22 (12.35%) sheep. The results obtained constitute the first molecular and serological evidence of A. phagocytophilum infection in sheep and cattle in the Black Sea Region of Turkey. © 2008 Akadémiai Kiadó.
Anahtar Kelimeler
cattle | sheep | Anaplasma phagocylophilum | prevalence | seroprevalence | indirect immunofluorescent antibody test (IFAT) | polymerase chain reaction (PCR)