Detection of cattle infected with Theileria annulata in fields by nestedPCR IFAT and microscopic examination of blood smears
Yazarlar (2)
Prof. Dr. Zati VATANSEVER Ankara Üniversitesi, Türkiye
Serpil Nalbantoǧlu Ankara Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Turkish Journal of Veterinary and Animal Sciences (Q4) | ||
| Dergi ISSN | 1300-0128 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2002 |
| Cilt / Sayı / Sayfa | 26 / 6 / 1465–1469 | DOI | 10.5555/20033001341 |
| Makale Linki | https://www.cabidigitallibrary.org/doi/full/10.5555/20033001341 | ||
| Özet |
| The aim of this study was to diagnose Theileria annulata infected cattle by microscopy, IFAT, and nested PCR and to compare the sensitivity and specificity of these diagnostic methods. A total of 147 blood and sera samples were collected from healthy cattle in 4 localities of Polatlı district, Ankara, Turkey, where tropical theileriosis is prevalent. Examination results revealed positivity rates of 31.3, 44.9, and 61.2% for microscopy, IFAT, and nested PCR, respectively. Nested PCR was more sensitive and specific compared to microscopical examination and serological findings. Moreover, nested PCR was more sensitive than single step PCR. This study, which is the first work utilizing nested PCR in the detection of T. annulata in Turkey, showed that nested PCR is more sensitive and specific when compared to microscopy and IFAT and can be used in further epidemiological studies. |
| Anahtar Kelimeler |
| Theileria annulata | PCR | nested PCR | IFAT | epidemiology |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 12 |
| SCOPUS | 13 |
| Google Scholar | 20 |
| Google Scholar | 15 |