Proinflammatory cytokine expression by Theileria annulata infected cell lines correlates with the pathology they cause in vivo
Yazarlar (10)
Simon P. Graham
The Royal (Dick) School Of Veterinary Studies, İngiltere
David J. Brown
University Of Edinburgh, Roslin Institute, İngiltere
Prof. Dr. Zati VATANSEVER
The Royal (Dick) School Of Veterinary Studies, İngiltere
David Waddington
University Of Edinburgh, Roslin Institute, İngiltere
Louise H. Taylor
The Royal (Dick) School Of Veterinary Studies, İngiltere
Anil K. Nichani
The Royal (Dick) School Of Veterinary Studies, İngiltere
John D.M. Campbell
The Royal (Dick) School Of Veterinary Studies, İngiltere
Rachel E. Adamson
University Of York, İngiltere
Elizabeth J. Glass
University Of Edinburgh, Roslin Institute, İngiltere
Roger L. Spooner
The Royal (Dick) School Of Veterinary Studies, İngiltere
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Vaccine (Q2) | ||
| Dergi ISSN | 0264-410X Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Exp, SCOPUS, Biological Abstracts, Biosis Full Coverage Shared, Biosis Previews, Curation, Current Contents Life Sciences, Derwent Drug File, Essential Science Indicators, Pdf2xml, Pdf2xml, Prous, Reference Master, Sophia | ||
| Makale Dili | İngilizce | Basım Tarihi | 04-2001 |
| Cilt / Sayı / Sayfa | 19 / 20 / 2932–2944 | DOI | 10.1016/S0264-410X(00)00529-6 |
| Makale Linki | http://www.ncbi.nlm.nih.gov/pubmed/11282205 | ||
| Özet |
| Control of Theileria annulata is currently best achieved by the use of live attenuated cell line vaccines. However, the mechanisms underlying attenuation are unclear and there is a need to rapidly produce new cell line vaccines, which could safely and effectively vaccinate cattle against tropical theileriosis. There is increasing evidence to suggest that proinflammatory cytokines produced by T. annulata infected cells play a central role in both pathology and immune evasion. This study aimed to test this hypothesis and to evaluate cytokine expression as a marker of virulence. The pathogenicity and protective efficacy of cloned T. annulata, cell lines that expressed different levels of proinflammatory cytokines were compared. In two independent trials using different stocks of T. annulata, cell lines that expressed higher levels of proinflammatory cytokines induced severe reactions, and in some cases death, when used to vaccinate groups of cattle. In contrast, low cytokine expressing lines induced low post-vaccinal reactions. The results clearly demonstrated that cytokine expression by T. annulata infected cells could be used as a marker of virulence and provided strong evidence to support a role for cytokines in the induction of pathology. Both high and low cytokine expressing cell lines protected cattle against heterologous challenge infection, offering the possibility of using cytokine expression to rapidly select new safe, potent vaccines against tropical theileriosis without the need for culture attenuation. © 2001 Elsevier Science Ltd. |
| Anahtar Kelimeler |
| Theileria annulata | proinflammatory cytokines | pathogenicity |
Science Direct
Proinflammatory cytokine expression by Theileria annulata infected cell lines correlates with the pathology they cause in vivo
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 41 |
| SCOPUS | 49 |
| Google Scholar | 72 |