| Yazarlar |
Çiğdem Gözde Aslan
Türkiye |
|
Hilmiye Deniz Ertuğrul Uygun
Türkiye |
Doç. Dr. Zihni Onur UYGUN
Türkiye |
|
Nalan Gülşen Ünal
Türkiye |
|
Yasemin Akçay
Türkiye |
| Özet |
| Crohn's disease, a chronic inflammatory disorder affecting the gastrointestinal tract, is on the rise, posing significant health challenges due to its potential long-term impact on intestinal health. Early and accurate detection of this disease is crucial for effective treatment. This study introduces an innovative approach to diagnosing Crohn's disease by utilizing a rapid and precise biosensor system as an alternative to conventional detection techniques. Utilization of an S100 A8 targeting aptamer for the specific identification of calprotectin, a well-established biomarker associated with Crohn's disease was used as a biorecognition receptor. The aptamer is immobilized on gold nanoparticles via cysteamine and avidin to construct a biosensor. The biosensor was compared with traditional quantitative lateral-flow tests (QLFA). The developed biosensor demonstrates that detection in 900 seconds. Performance parameters, including a limit of detection (LOD) as 5.57 μg/g and a limit of quantification (LOQ) as 16.89 μg/g, are determined through electrochemical impedance spectroscopy (EIS). Accordingly, the calibration curve equation was calculated as y=3.9736x + 1570.4 and the regression coefficient was found as R2=0.9917. These results showed that biosensor‘s capability to reliably measure calprotectin levels in real samples. |
| Anahtar Kelimeler |
| biosensor,calprotectin,chronoimpedance,Crohn's disease,impedance |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Wiley |
| Dergi ISSN | 1040-0397 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | Türkçe |
| Basım Tarihi | 09-2023 |
| Cilt No | 35 |
| Sayı | 12 |
| Sayfalar | 1 / 7 |
| Doi Numarası | 10.1002/elan.202300119 |
| Makale Linki | http://dx.doi.org/10.1002/elan.202300119 |
| Atıf Sayıları | |
| WoS | 1 |
| SCOPUS | 1 |
| Google Scholar | 1 |
