Label-free highly sensitive detection of DNA approximate length and concentration by impedimetric CRISPR-dCas9 based biosensor technology
Yazarlar (2)
Doç. Dr. Zihni Onur UYGUN Kafkas Üniversitesi, Türkiye
Doç. Dr. Sevcan Atay Ege University Medical School, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Bioelectrochemistry (Q1) | ||
| Dergi ISSN | 1567-5394 Dergi Bilgileri (2021) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 08-2021 |
| Cilt / Sayı / Sayfa | 140 / 1 / 107812–0 | DOI | 10.1016/j.bioelechem.2021.107812 |
| Makale Linki | http://dx.doi.org/10.1016/j.bioelechem.2021.107812 | ||
| UAK Araştırma Alanları |
Tıbbi Biyokimya
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| Özet |
| In this study, we designed a CRISPR-dCas9-based biosensor with potential clinical use in glioblastoma subtype discrimination through detection of isocitrate dehydrogenase R132H (IDH) mutation status. The electrode was modified to detect mutant DNA cysteamine (Cys), PAMAM, dCas9 and sgRNA for R132H mutations, respectively. The biosensor system we proposed was able not only to detect mutant DNA, but also to measure the approximate length of DNA. Therefore, it can be considered that the biosensor technology that we developed is novel in the field of DNA biosensors. Another superior capability of the biosensor system is that it can simultaneously measure DNA concentration by electrochemical impedance spectroscopy and DNA length by capacitive detection, which lowers the concentration-based false-positive signals. The calibration range was obtained between 100 fM and 1000 fM, LOD and LOQ ... |
| Anahtar Kelimeler |
| Biosensor | Capacitance | Chronoimpedance | CRISPR | dCas9 | Electrochemical impedance spectroscopy |
Science Direct
Label-free highly sensitive detection of DNA approximate length and concentration by impedimetric CRISPR-dCas9 based biosensor technology
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 17 |
| Scopus | 25 |
| Google Scholar | 26 |