| Yazarlar |
Cemrehan Fedacı
Türkiye |
|
Hilmiye Deniz Ertuğrul Uygun
Türkiye |
Doç. Dr. Zihni Onur UYGUN
Türkiye |
|
Yasemin Akçay
Ege Üniversitesi, Türkiye |
| Özet |
| In this study, Citropin-A (Cit-A) as a biorecognition receptor was used for the first time to develop electrochemical impedance spectroscopy (EIS) based biosensor for the detection of Lymph Node Carcinoma of the Prostate (LNCaP) cancer cells. The biosensor was engineered by modification of a gold electrode (AuE) with cysteamine (Cys), Poliamidoamin (PAMAM (G4)) dendrimers, avidin, and biotinylated Cit-A, respectively. The detection time of the LNCaP cells was determined as 300 s by chronoimpedance (CI). Chronoimpedance also provided an exact detection time to avoid non-specific adsorptions. The biosensor showed good linearity between 1500 cells/L and 12000 cells/L, limit of detection (LOD), and limit of quantification (LOQ) values were 518 cells/L and 1570 cells/L, respectively. |
| Anahtar Kelimeler |
| Impedance | Chronoimpedance | Biosensor | Antimicrobial peptide | Prostate cancer |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | ANALYTICAL BIOCHEMISTRY |
| Dergi ISSN | 0003-2697 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 09-2022 |
| Cilt No | 652 |
| Sayı | 1 |
| Sayfalar | 114772 / 0 |
| Doi Numarası | 10.1016/j.ab.2022.114772 |
| Makale Linki | http://dx.doi.org/10.1016/j.ab.2022.114772 |
| Atıf Sayıları | |
| WoS | 1 |
| SCOPUS | 3 |
| Google Scholar | 4 |
