Rapid Fibroblast Cell Culture Characterization with Impedimetric Label‐free Heat Shock Protein – 47 Biosensor
Yazarlar (6)
Duygu Harmancı
Dokuz Eylül Üniversitesi, Türkiye
Doç. Dr. Zihni Onur UYGUN
Kafkas Üniversitesi, Türkiye
Ayşe Koçak Sezgin
Dokuz Eylül Üniversitesi, Türkiye
Cenk Demirdöver
Dokuz Eylül Üniversitesi, Türkiye
Ferhan Girgin Sağın
Ege University Medical School, Türkiye
Gül Akdoğan
Izmir Ekonomi Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Electroanalysis | ||
| Dergi ISSN | 1040-0397 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 10-2020 |
| Cilt / Sayı / Sayfa | 32 / 10 / 2310–2315 | DOI | 10.1002/elan.202060264 |
| Makale Linki | https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.202060264 | ||
| Özet |
| In this study, for the first time in literature, an impedimetric biosensor technology was developed to determine Heat Shock Protein-47 (HSP-47) for the characterization of primary skin fibroblast cells. Gold nanoparticle electrodes (GNPE) were modified with amino-functionalized graphene oxide layers and anti-HSP-47 were immobilized on this layer. The performance of the biosensor was tested with real samples as cell lysates. The low detection limit (LOD) and the linear detection limit (LOQ) of the biosensor were calculated as 9.47 pg/mL and 3.23 pg/mL, respectively. Linear measurement range was obtained between 10–160 pg/mL HSP-47. The reproducibility of the biosensor was good. |
| Anahtar Kelimeler |
| HSP-47 | impedance | gold nanoparticle | graphene | chronoimpedance |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 3 |
| SCOPUS | 3 |
| Google Scholar | 3 |