Rapid Fibroblast Cell Culture Characterization with Impedimetric Label‐free Heat Shock Protein – 47 Biosensor
Yazarlar (6)
Duygu Harmancı Dokuz Eylül Üniversitesi, Türkiye
Doç. Dr. Zihni Onur UYGUN Kafkas Üniversitesi, Türkiye
Doç. Dr. Ayşe Koçak Sezgin Kütahya Sağlık Bilimleri Üniversitesi, Türkiye
Cenk Demirdöver Dokuz Eylül Üniversitesi, Türkiye
Prof. Dr. Ferhan Girgin Sağın Ege Üniversitesi, Türkiye
Gül Akdoğan İzmir Ekonomi Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Electroanalysis (Q2)
Dergi ISSN 1040-0397 Dergi Bilgileri (2020)
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 01-2020
Cilt / Sayı / Sayfa 32 / 10 / 2310–2315 DOI 10.1002/elan.202060264
Makale Linki https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.202060264
UAK Araştırma Alanları
Tıbbi Biyokimya
Özet
In this study, for the first time in literature, an impedimetric biosensor technology was developed to determine Heat Shock Protein‐47 (HSP‐47) for the characterization of primary skin fibroblast cells. Gold nanoparticle electrodes (GNPE) were modified with amino‐functionalized graphene oxide layers and anti‐HSP‐47 were immobilized on this layer. The performance of the biosensor was tested with real samples as cell lysates. The low detection limit (LOD) and the linear detection limit (LOQ) of the biosensor were calculated as 9.47 pg/mL and 3.23 pg/mL, respectively. Linear measurement range was obtained between 10–160 pg/mL HSP‐47. The reproducibility of the biosensor was good.
Anahtar Kelimeler
chronoimpedance | gold nanoparticle | graphene | HSP-47 | impedance
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Web of Science 4
Scopus 4
Google Scholar 4
Rapid Fibroblast Cell Culture Characterization with Impedimetric Label‐free Heat Shock Protein – 47 Biosensor

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