| Yazarlar |
Doç. Dr. Zihni Onur UYGUN
Kafkas Üniversitesi, Türkiye |
Ferhan Girgin Sağın
Ege Üniversitesi, Türkiye |
| Özet |
| Objectives: Sensitive and accurate techniques for early detection of prostate cancer, which has a good chance for successful treatment if detected early, are of utmost value. Our aim is to develop a sensitive chronoimpedimetric biosensor for detection of circulating prostatic tumor cells (CTCs) with an aptamer selective for prostate specific membrane antigen (PSMA). Methods: Thiolated PSMA-specific aptamer was immobilized on the gold nanoparticle modified carbon screen-printed electrodes. After characterization with cyclic voltammetry and electrochemical impedance spectrometry, scanning electron microscopy and atomic force microscopy studies were conducted to confirm the modifications. LNCaP cells (androgen-sensitive human prostate adenocarcinoma cells), were then added to the serum samples and chronoimpedimetric detection of CTCs in samples were performed. Results: Our study showed one cell detection capability in real serum samples with a linear range from 1 to 40 cells/ mL. The incubation time was 130 s. LOD was found to be 0.62 cells/mL and relative standard deviations were lower than 2% RSD. Reproducibility tests indicated a regression coefficient as R2 = 0.9963 ± 0.0178. Conclusions: This new biosensor enables rapid, accurate, precise, reproducible and highly sensitive detection of PSMA on CTCs in prostate cancer and paves the way to new diagnostic applications and research-based studies. |
| Anahtar Kelimeler |
| aptasensor | chronoimpedance | CTC | liquid biopsy | prostate cancer | PSMA | aptasensor | kronoimpedans | PSMA | CTC | sivi biyopsi | prostat kanseri |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI |
| Dergi ISSN | 0250-4685 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2021 |
| Cilt No | 46 |
| Sayı | 6 |
| Sayfalar | 631 / 637 |
| Doi Numarası | 10.1515/tjb-2021-0056 |
| Makale Linki | http://dx.doi.org/10.1515/tjb-2021-0056 |
| Atıf Sayıları | |
| WoS | 4 |
| SCOPUS | 8 |
| Google Scholar | 9 |
