Impedimetric Detection of Albumin-Bound Fatty Acids Using Graphene Oxide Electrode
Yazarlar (3)
Doç. Dr. Zihni Onur UYGUN Kafkas Üniversitesi, Türkiye
Prof. Dr. Soner Duman Ege University Medical School, Türkiye
Prof. Dr. İsmail Oran Ege University Medical School, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Chemosensors (Q1) | ||
| Dergi ISSN | 2227-9040 Dergi Bilgileri (2021) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 08-2021 |
| Cilt / Sayı / Sayfa | 9 / 9 / 240–0 | DOI | 10.3390/chemosensors9090240 |
| Makale Linki | https://doi.org/10.3390/chemosensors9090240 | ||
| UAK Araştırma Alanları |
Tıbbi Biyokimya
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| Özet |
| The fatty acid/albumin (FA/Alb) molar ratio is ≤1 in healthy subjects; this ratio can reach 3--4 in patients with acute myocardial ischemia. We describe the spontaneous desorption--adsorption kinetics of FAs from albumin to a graphene electrode at neutral pH. Albumin-depleted human serum was prepared via ultrafiltration and then mixed with defatted human albumin and sodium oleate at different FA/Alb molar ratios, at a final albumin concentration of 0.6 mM. A commercially available screen-printed graphene oxide (GO)-modified carbon electrode was used for the electrochemical experiments. Frequency-ranged Faradaic electrochemical impedance spectroscopy (EIS) and a single-frequency non-Faradaic impedance measure (chronoimpedance) were used to derive the desorption--adsorption kinetics. The surface of the GO electrode was finally evaluated with the aid of X-ray photoelectron spectroscopy (XPS). With the chronoimpedance experiment, the measured impedance increased accordingly to the FA/Alb ratios. The frequency-ranged EIS showed good linearity between the impedance and the FA/Alb ratio, with a limit of quantification value of 1.06. XPS surface analysis revealed that the FA was adsorbed onto the electrode, with the amount of the adsorbed FA proportional to the FA/Alb ratio. The electrochemical method applied on this peculiar desorption--adsorption kinetics of FAs has the ability to differentiate serum having excess FAs. |
| Anahtar Kelimeler |
| Albumin | Chronoimpedance | Fatty acid | Graphene | Impedance spectroscopy |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 2 |
| Scopus | 3 |
| Google Scholar | 3 |