| Yazarlar |
Doç. Dr. Zihni Onur UYGUN
Kafkas Üniversitesi, Türkiye |
Soner Duman
Türkiye |
|
İsmail Oran
Türkiye |
| Özet |
| The fatty acid/albumin (FA/Alb) molar ratio is ≤1 in healthy subjects; this ratio can reach 3–4 in patients with acute myocardial ischemia. We describe the spontaneous desorption–adsorption kinetics of FAs from albumin to a graphene electrode at neutral pH. Albumin-depleted human serum was prepared via ultrafiltration and then mixed with defatted human albumin and sodium oleate at different FA/Alb molar ratios, at a final albumin concentration of 0.6 mM. A commercially available screen-printed graphene oxide (GO)-modified carbon electrode was used for the electrochemical experiments. Frequency-ranged Faradaic electrochemical impedance spectroscopy (EIS) and a single-frequency non-Faradaic impedance measure (chronoimpedance) were used to derive the desorption–adsorption kinetics. The surface of the GO electrode was finally evaluated with the aid of X-ray photoelectron spectroscopy (XPS). With the chronoimpedance experiment, the measured impedance increased accordingly to the FA/Alb ratios. The frequency-ranged EIS showed good linearity between the impedance and the FA/Alb ratio, with a limit of quantification value of 1.06. XPS surface analysis revealed that the FA was adsorbed onto the electrode, with the amount of the adsorbed FA proportional to the FA/Alb ratio. The electrochemical method applied on this peculiar desorption–adsorption kinetics of FAs has the ability to differentiate serum having excess FAs. |
| Anahtar Kelimeler |
| graphene | fatty acid | albumin | chronoimpedance | impedance spectroscopy |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | CHEMOSENSORS |
| Dergi ISSN | 2227-9040 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 09-2021 |
| Cilt No | 9 |
| Sayı | 9 |
| Sayfalar | 240 / 0 |
| Doi Numarası | 10.3390/chemosensors9090240 |
| Makale Linki | https://doi.org/10.3390/chemosensors9090240 |
| Atıf Sayıları | |
| WoS | 1 |
| SCOPUS | 2 |
| Google Scholar | 3 |
