| Yazarlar |
Doç. Dr. Zihni Onur UYGUN
Kafkas Üniversitesi, Türkiye |
Mustafa Kemal Sezgintürk
Namık Kemal Üniversitesi, Türkiye |
| Özet |
| In the presented research, a novel, ultra sensitive biosensor for the impedimetric detection of vascular endothelial growth factor (VEGF) is introduced. The human vascular endothelial growth factor receptor 1 (VEGF-R1, Flt-1) was used as a biorecognition element for the first time. The immobilization of VEGF-R1 on glassy carbon electrodes was carried out using layer-by-layer covalent attachment of VEGFR1. The electrochemical properties of the layers constructed on the electrodes were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The differences in electron transfer resistance (Ret) between the working solution and the biosensor surface, recorded by the redox probe K3[Fe(CN)6]/K4[Fe(CN)6], confirmed the binding of VEGF to VEGF-R1. The new biosensor allowed a detection limit of 100 fg mL-1 with a linear range of 100-600 fg mL-1 to be obtained. The biosensor also exhibited good repeatability (with a correlation coefficient of 1.95%), and reproducibility. © 2011 Elsevier B.V. |
| Anahtar Kelimeler |
| Tumor growth,Cancer,Vascular endothelial growth factor,Vascular endothelial growth factor receptor-1,Electrochemical impedance spectroscopy,Biosensor |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Analytica Chimica Acta |
| Dergi ISSN | 0003-2670 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 11-2011 |
| Cilt No | 706 |
| Sayı | 2 |
| Sayfalar | 343 / 348 |
| Doi Numarası | 10.1016/j.aca.2011.08.044 |
| Makale Linki | http://dx.doi.org/10.1016/j.aca.2011.08.044 |
| Atıf Sayıları | |
| WoS | 20 |
| SCOPUS | 22 |
| Google Scholar | 34 |
