| Yazarlar |
Mustafa Kemal Sezgintürk
Namık Kemal Üniversitesi, Türkiye |
Doç. Dr. Zihni Onur UYGUN
Kafkas Üniversitesi, Türkiye |
| Özet |
| In the present work, we have developed a new biosensor based on fourth-generation (G4) PAMAM dendrimers for the analysis of vascular endothelial growth factor (VEGF). First, the PAMAM dendrimers were covalently attached to a cysteamine-modified Au electrode by glutaraldehyde. With the help of the amino groups located on its surface, vascular endothelial growth factor receptor-1 (VEGF-R1) was immobilized via glutaraldehyde cross-linking. VEGF-R1 loading was investigated to identify the optimal VEGF-R1 immobilization conditions for the best sensitivity of the new biosensor. In addition, Kramers-Kronig transforms were also analyzed for immobilization and measurement processes. The biosensor had a linear range of 5 to 125 pg/mL VEGF. The fabricated biosensor had good repeatability and reproducibility. Finally, the results for artificial serum samples measured by the present biosensor showed a good recovery for VEGF detection. © 2012 Elsevier Inc. All rights reserved. |
| Anahtar Kelimeler |
| VEGF,Tumor growth,VEGF-R1,PAMAM,Electrochemical impedance spectroscopy,Biosensor |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Analytical Biochemistry |
| Dergi ISSN | 0003-2697 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 04-2012 |
| Cilt No | 423 |
| Sayı | 2 |
| Sayfalar | 277 / 285 |
| Doi Numarası | 10.1016/j.ab.2011.12.049 |
| Makale Linki | http://dx.doi.org/10.1016/j.ab.2011.12.049 |
| Atıf Sayıları | |
| WoS | 25 |
| SCOPUS | 28 |
| Google Scholar | 34 |
