An impedimetric vascular endothelial growth factor biosensor-based PAMAM/cysteamine-modified gold electrode for monitoring of tumor growth
Yazarlar (2)
Prof. Dr. Mustafa Kemal Sezgintürk Namık Kemal Üniversitesi, Türkiye
Doç. Dr. Zihni Onur UYGUN Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Analytical Biochemistry (Q1) | ||
| Dergi ISSN | 0003-2697 Dergi Bilgileri (2012) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 04-2012 |
| Cilt / Sayı / Sayfa | 423 / 2 / 277–285 | DOI | 10.1016/j.ab.2011.12.049 |
| Makale Linki | http://dx.doi.org/10.1016/j.ab.2011.12.049 | ||
| UAK Araştırma Alanları |
Elektrokimya
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| Özet |
| In the present work, we have developed a new biosensor based on fourth-generation (G4) PAMAM dendrimers for the analysis of vascular endothelial growth factor (VEGF). First, the PAMAM dendrimers were covalently attached to a cysteamine-modified Au electrode by glutaraldehyde. With the help of the amino groups located on its surface, vascular endothelial growth factor receptor-1 (VEGF-R1) was immobilized via glutaraldehyde cross-linking. VEGF-R1 loading was investigated to identify the optimal VEGF-R1 immobilization conditions for the best sensitivity of the new biosensor. In addition, Kramers–Kronig transforms were also analyzed for immobilization and measurement processes. The biosensor had a linear range of 5 to 125pg/mL VEGF. The fabricated biosensor had good repeatability and reproducibility. Finally, the results for artificial serum samples measured by the present biosensor showed a good … |
| Anahtar Kelimeler |
| Biosensor | Electrochemical impedance spectroscopy | PAMAM | Tumor growth | VEGF | VEGF-R1 |
Science Direct
An impedimetric vascular endothelial growth factor biosensor-based PAMAM/cysteamine-modified gold electrode for monitoring of tumor growth
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 26 |
| Scopus | 31 |
| Google Scholar | 39 |