CRISPR-dCas9 powered impedimetric biosensor for label-free detection of circulating tumor DNAs
Yazarlar (3)
Doç. Dr. Zihni Onur UYGUN Kafkas Üniversitesi, Türkiye
Levent Yeniay
Ege Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
Prof. Dr. Ferhan Girgin Sağın Ege Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Analytica Chimica Acta (Q1) | ||
| Dergi ISSN | 0003-2670 Dergi Bilgileri (2020) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 07-2020 |
| Cilt / Sayı / Sayfa | 1121 / 1 / 35–41 | DOI | 10.1016/j.aca.2020.04.009 |
| Makale Linki | http://dx.doi.org/10.1016/j.aca.2020.04.009 | ||
| UAK Araştırma Alanları |
Tıbbi Biyokimya
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| Özet |
| Label-free biosensors which can be integrated into lab-on-a-chip platforms have the advantage of using small volumes for rapid and inexpensive measurements contrary to label-based technologies which are often more costly and time-consuming. In this study, graphene oxide screen printed electrodes (GPHOXE) were modified by deactivated Cas9 (dCas9) proteins and synthetic guide RNA (sgRNA) as the biorecognition receptor for label-free detection of circulating tumor DNAs (ctDNA). This was achieved by detection of a tumor related mutation (PIK3CA exon 9 mutation) via sequence-specific recognition followed by electrochemical impedance spectroscopy (EIS) analysis. The biosensor showed high specificity as there was no impedance signal for other ctDNA sequences, even the single nucleotide mismatch. dCas9-sgRNA modified biosensor demonstrated linear detection limits between 2 and 20 nM for 120 ... |
| Anahtar Kelimeler |
| Cancer diagnosis | Chronoimpedance | Circulating tumor DNA | CRISPR/Cas9 technology | Deactivated Cas9 | Electrochemical impedance spectroscopy | Impedimetric biosensor | Label-free analysis | Liquid biopsy |
Science Direct
CRISPR-dCas9 powered impedimetric biosensor for label-free detection of circulating tumor DNAs
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 108 |
| Scopus | 117 |
| Google Scholar | 128 |