CRISPR-dCas9 powered impedimetric biosensor for label-free detection of circulating tumor DNAs
Yazarlar (3)
Doç. Dr. Zihni Onur UYGUN Kafkas Üniversitesi, Türkiye
Levent Yeniay Ege Üniversitesi, Türkiye
Ferhan Girgin Sağın Ege Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Analytica Chimica Acta (Q1) | ||
| Dergi ISSN | 0003-2670 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 07-2020 |
| Cilt / Sayı / Sayfa | 1121 / 1 / 35–41 | DOI | 10.1016/j.aca.2020.04.009 |
| Makale Linki | http://dx.doi.org/10.1016/j.aca.2020.04.009 | ||
| Özet |
| Label-free biosensors which can be integrated into lab-on-a-chip platforms have the advantage of using small volumes for rapid and inexpensive measurements contrary to label-based technologies which are often more costly and time-consuming. In this study, graphene oxide screen printed electrodes (GPHOXE) were modified by deactivated Cas9 (dCas9) proteins and synthetic guide RNA (sgRNA) as the biorecognition receptor for label-free detection of circulating tumor DNAs (ctDNA). This was achieved by detection of a tumor related mutation (PIK3CA exon 9 mutation) via sequence-specific recognition followed by electrochemical impedance spectroscopy (EIS) analysis. The biosensor showed high specificity as there was no impedance signal for other ctDNA sequences, even the single nucleotide mismatch. dCas9-sgRNA modified biosensor demonstrated linear detection limits between 2 and 20 nM for 120 … |
| Anahtar Kelimeler |
| Cancer diagnosis | Chronoimpedance | Circulating tumor DNA | CRISPR/Cas9 technology | Deactivated Cas9 | Electrochemical impedance spectroscopy | Impedimetric biosensor | Label-free analysis | Liquid biopsy |
Science Direct
CRISPR-dCas9 powered impedimetric biosensor for label-free detection of circulating tumor DNAs
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 124 |
| Web of Science | 106 |
| Scopus | 116 |