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Tracking the footsteps of Francisella tularensis: Bacteriological and serological monitoring in epidemic areas in Ankara    
Yazarlar
Derya Karataş Yeni
Türkiye
 Fatih BÜYÜK Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye
Mehmet Engin Malal
Türkiye
Salahuddin Shah
Türkiye
Asma Ashraf
Türkiye
Özet
The study aimed to detect Francisella tularensis (F. tularensis) in water samples and to investigate the seroreactivity of sheep to tularemia in endemic areas where human tularemia cases have been reported in Ankara, Turkey. For the isolation of F. tularensis, 50 water samples were collected from rural areas of 5 regions of Ankara (Turkey) and selectively cultured on Francis medium supplemented with 8–9 % sheep blood and antibiotics (100 IU/ml penicillin G, 100 mg/L cycloheximide, 80,000 U/L polymixin B). No F. tularensis isolate was cultivated from the water samples. To determine the seroreactivity of sheep to tularemia, 1006 sheep blood samples were collected from the regions, where human tularemia is endemic. A microagglutination test (MAT) identified significant antibody titers, ranging from 1/20–1/640 in 181 (17.99 %) of the investigated sheep sera. Further investigation is required in order to evaluate and confirm a possible epidemiologic relationship between human outbreaks and probable role of sheep or other sources.
Anahtar Kelimeler
Culture | MAT | Serology | Sheep | Tularemia | Water
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Comparative Immunology, Microbiology and Infectious Diseases
Dergi ISSN 0147-9571
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q2
Makale Dili İngilizce
Basım Tarihi 01-2023
Cilt No 92
Sayı 1
Doi Numarası 10.1016/j.cimid.2022.101921
Makale Linki https://www.sciencedirect.com/science/article/pii/S0147957122001783